Dendrimer-Like Polysaccharides: Structure, Modification, and Functional Colloidal Assembly

该奖项由普渡大学材料研究部生物材料项目颁发，旨在研究植物糖原（PG）纳米颗粒的结构-功能关系，并确定PG纳米颗粒介导的胶体系统工程的关键因素。生物基纳米材料在食品安全和营养、药物输送、个人护理和农业工程等领域具有巨大的潜力。本项目将以PG为原料制备一类新型的生物基树突状多糖纳米材料，其粒径为30-100 nm，分子密度极高。在与两亲性基团接枝后，PG可用于形成功能性胶体组装体。这些组件的一个突出特性是保护和传递亲脂化合物，如ω -3脂肪酸。该项目的目的是揭示将从su1突变体玉米中提取的天然PG，进行分选，并进行化学和酶修饰。功能化PG纳米颗粒的性质将被评估其实现增强氧化稳定性和控制脂质的释放的能力。在这个项目的支持下，将开发一个教学模块“碳水化合物设计前沿”，并将其纳入pi的教育活动中。这一努力将极大地有利于本科生和研究生，以及参加普渡大学年度碳水化合物短期课程的工业人员。在这个项目中，从玉米突变体中提取了一种碳水化合物聚合物，植物糖原（PG），并将其转化为功能性纳米颗粒。PG是由密集分布的树枝组成的树突状多糖。基于植物糖原的纳米颗粒是可消化和可生物降解的，这使得它们能够在生理条件下传递生物活性化合物。例如，这些纳米颗粒可以保护脂质营养素（如ω -3脂肪酸）免受氧化，并在消化过程中释放它们。在基础层面上，该项目将建立一种新的战略，用于生产可持续、无害环境和经济的纳米结构，这将有利于食品安全和营养、药物输送、个人护理和农业工程等领域。在本项目的支持下，将开发一个教学模块，介绍碳水化合物科学的前沿发展，包括碳水化合物改性、碳水化合物在食品安全和营养中的应用、碳水化合物为基础的输送系统、碳水化合物与绿色化学。该模块将被纳入校园的教育活动中，用于培训本科生和研究生，以及参加普渡大学年度“碳水化合物短期课程”的工业人员。

This award by the Biomaterials program in the Division of Materials Research to Purdue University is to study structure-function relationship of phytoglycogen (PG) nanoparticles and identify key factors for engineering PG nanoparticle-mediated colloidal systems. Biobased nanomaterials have enormous potential in the areas of food safety and nutrition, drug delivery, personal care, and agricultural engineering. In this project, a new class of biobased dendrimer-like polysaccharide nanomaterials will be prepared from PG, which has a particle size of 30-100 nm and an exceptionally high molecular density. After being grafted with amphiphilic groups, PG can be used to form functional colloidal assemblies. An outstanding property of these assemblies is to protect and deliver lipophilic compounds, such as omega-3 fatty acids. The aim of this project is to reveal the native PG that will be extracted from the su1 mutant maize, fractionated, and subjected to chemical and enzymatic modifications. The properties of functionalized PG nanoparticles will be evaluated for their capability to realize enhanced oxidative stability and controlled release of lipids. Supported by this project, a teaching module 'Frontiers in Carbohydrate Design' will be developed and incorporated into the PIs' educational activities. This effort will greatly benefit the undergraduate and graduate students involved, as well as the industrial personnel attending the annual Carbohydrate Short Course at Purdue University.In this project, a carbohydrate polymer, phytoglycogen (PG), is extracted from a corn mutant and transformed into functional nanoparticles. PG is a dendrimer-like polysaccharide made from densely distributed branches. Phytoglycogen-based nanoparticles are digestible and biodegradable, which allows them to deliver bioactive compounds in physiological conditions. For example, these nanoparticles may protect lipid nutrients (e.g. omega-3 fatty acids) from oxidation and release them during digestion. At the fundamental level, this project will establish a new strategy for generating sustainable, environmentally benign, and economical nano-constructs that will benefit the areas of food safety and nutrition, drug delivery, personal care, and agricultural engineering. Supported by this project, a teaching module will be developed to introduce the cutting-edge development of carbohydrate science, including carbohydrate modifications, carbohydrates in food safety and nutrition, carbohydrate-based delivery systems, and carbohydrates and green chemistry. This module will be incorporated into educational activities in the campus for training undergraduate and graduate students, as well as the industrial personnel attending the annual 'Carbohydrate Short Course' at Purdue University.