STTR Phase I: Development of a novel BioNematicide to control soybean cyst nematode

这个小企业技术转让（STTR）第一阶段项目提出开发一种新型生物杀线虫剂来控制大豆中的大豆胞囊线虫（SCN）。 在广泛的初步研究中，这种生物杀线虫剂控制了几个线虫阶段，包括高达90%的孢囊（SCN侵染的主要接种源）。 该分离物还在广泛的土壤温度和SCN小种（生物型）范围内控制SCN，表明SCN对分离物的抗性将产生最小的影响。 该项目的总体目标是：1）演示和确定应用该技术的现场参数; 2）评估最终商业生产的规模扩大生产方案。 该项目的优势是通过一个强大的公共和私人联盟利用非常多样化的专业知识的协作努力得到支持。 该联盟提供了一个很好的框架来设计和实施现场和实验室测试以及营销（和相关）策略，以使项目成功。 研究结果将为快速商业化提供数据和结构。该项目更广泛的影响/商业潜力是，SCN是世界上最重要的大豆经济害虫之一。 大多数化学杀线虫剂已被去除或很少使用，抗性品种继续分解为SCN生物型，由于适用作物的经济和环境限制，在大多数地区轮作是不可行的。 基于这些因素，该技术一旦商业化，将在市场上产生重大影响。 仅在美国，就有50，000，000英亩（并且还在增长）的大豆受到SCN的侵扰，估计每年给农民造成15亿美元的损失。而且，由于该产品的环境（或“绿色”）无害和可持续性质，该技术将通过全面减少化学农药的应用并通过提供大豆孢囊管理中急需的选择，为消费公众创造更广泛的影响。 这种主要害虫迫切需要有效、可持续的防治方法，如本提案中概述的方法，这将创造重大的市场机会和社会效益。

This Small Business Technology Transfer (STTR) Phase I project proposes to develop a novel bionematicide to control soybean cyst nematode (SCN) in soybeans. In wide-ranging preliminary research, this bionematicide controlled several nematode stages, including up to 90% of cysts (the primary inoculum source for SCN infestations). The isolate also controls SCN over a broad range of soil temperatures and SCN races (biotypes), indicating that resistance to the isolate by SCN will be of minimal consequence. The overall objectives of this project are to 1) demonstrate and determine field parameters for application of the technology and 2) evaluate scale-up production protocol(s) for eventual commercial production. The advantage of the project is supported through the collaborative efforts of a strong public and private alliance utilizing very diverse professional expertise. This alliance provides an excellent framework to design and implement field and laboratory testing as well as marketing (and related) strategies to make the project successful. Results will provide data and structure for an expeditious commercialization track.The broader impact/commercial potential of this project is that SCN is one of the most economically important disease pest of soybeans in the world. Most chemical nematicides have been removed or are little used, resistant cultivars continue to break down to SCN biotypes, and crop rotation is not feasible in most areas due to economic and environmental constraints of applicable crops. Based on these factors the technology, once commercialized, will have a significant impact in the market. In the U.S. alone, there are 50,000,000 soybean acres (and growing) infested with SCN, costing farmers an estimated $1.5 billion annually. And, because of the environmentally (or "green") sound and sustainable nature of the product, the technology will create much broader impacts for the consuming public through the overall reduction of chemical pesticide applications and by providing for a much needed option in soybean cyst management. Effective, sustainable control methods such as outlined in this proposal are badly needed for this major pest creating a significant market opportunity as well as societal benefits.