Gel-Free DNA Separations in Micro/Nanocapillaries: Modeling and Applications

微/纳米毛细管中的无凝胶 DNA 分离：建模和应用

• 批准号: 1011957
• 负责人: Shaorong Liu
• 金额: $ 37.5万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1011957&HistoricalAwards=false
• 关键词: Gel; Free; DNA; Separations; Micro

With support from the Chemical Measurement and Imaging Program, Prof. Shaorong Liu and his group at the University of Oklahoma are seeking better understanding of how a narrow bore capillary can be used to effectively separate DNA fragments (from 75 to 106,000 base pairs) and proteins with efficiencies comparable to those achieved with capillary zone electrophoresis. The work entails systematic investigation to elucidate the separation mechanisms and their relative contributions. Development of separation techniques using narrow capillaries will extend the limits of separation science - critical to research in biological, biotechnological, chemical, pharmaceutical, environmental, geochemical, and agricultural disciplines. The inherently interdisciplinary nature of this research requires new modes of student training and new curricula incorporating aspects of theory, computation, nanofluidics, and separations. Substantial education and recruitment activities in nanoscience and technology are particularly aimed at groups underrepresented in science and engineering, including recruitment through a regional network of primarily undergraduate institutions, many of which have large populations strong in underrepresented talent. Results will be incorporated into general-public and high-school level nanoscience seminar series being developed by the PI in conjunction with activists at the Norman library.

在化学测量和成像项目的支持下，俄克拉荷马州大学的刘绍荣教授和他的团队正在寻求更好地了解如何使用窄孔毛细管有效地分离DNA片段（从75到106，000个碱基对）和蛋白质，其效率与毛细管区带电泳相当。这项工作需要系统的调查，以阐明分离机制和它们的相对贡献。使用窄毛细管的分离技术的发展将扩展分离科学的极限-对生物学、生物技术、化学、制药、环境、地球化学和农业学科的研究至关重要。这项研究固有的跨学科性质需要新的学生培训模式和新的课程，包括理论，计算，纳米流体和分离方面。纳米科学和技术的大量教育和招聘活动特别针对科学和工程领域代表性不足的群体，包括通过主要是本科院校的区域网络进行招聘，其中许多院校拥有大量代表性不足的人才。 结果将被纳入普通公众和高中水平的纳米科学研讨会系列正在开发的PI与活动家在诺曼图书馆。