Biotechnology of Supramolecular Complexes of Pyrene-Based Fluorescent Dyes

弗吉尼亚联邦大学化学系的Vladimir Sidorov博士和他的同事们发现了一个水溶性阴离子染料的基于循环素的受体家族。在美国国家科学基金会化学部大分子、超分子和纳米化学项目的支持下，Sidorov小组正在开发基于周期蛋白受体的技术，用于监测生物膜过程。将设计和合成结合阴离子染料和与生物相关底物共轭的染料的循环素受体。基于这些受体的技术将用于膜渗漏测定、脂质体含量的细胞内运输、药物传递系统分析和肝素低聚物分离。这项研究采用超分子化学技术来监测生物膜过程和分离生物活性化合物，将对人类治疗产生影响，并广泛影响生物有机和生物群落。这个项目的研究、培训和教育活动将有助于人力资源的发展。

Dr. Vladimir Sidorov and coworkers at the Chemistry Department of Virginia Commonwealth University have identified a family of cyclen-based receptors for water-soluble anionic dyes. With the support from the Macromolecular, Supramolecular, and Nanochemistry Program in the Division of Chemistry at NSF, the Sidorov group is developing techniques based on cyclen-based receptors for monitoring biomembrane processes. Cyclen-based receptors that bind anionic dyes and dyes conjugated to biologically-relevant substrates will be designed and synthesized. Techniques based on these receptors will be developed for membrane leakage assay, intra-cellular trafficking of the liposomal content, the analysis of drug delivery systems, and the separation of heparin oligomers.This research, which employs supramolecular chemistry in techniques for monitoring biomembrane processes and for separating bioactive compounds, would have implications for human therapeutics and broadly impact the bioorganic and biological communities. The research training and educational activities of this project would contribute to human resource development.