SBIR Phase I: In vivo Fluorescence Imaging Kit for Cell Proliferation.

SBIR 第一阶段：用于细胞增殖的体内荧光成像试剂盒。

• 批准号: 1013513
• 负责人: Romina Mancusso
• 金额: $ 15万
• 依托单位: MBMR Biolabs Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1013513&HistoricalAwards=false
• 关键词: SBIR; Phase; vivo; Fluorescence; Imaging

This Small Business Innovation Research (SBIR) Phase I project is to apply novel bio-orthogonal tetrazine ligation technology to fluorescent labeling of DNA replication, and to ultimately produce a fluorescence imaging kit (FIK) for cell proliferation. The advantages are two-fold: 1) Because of the novel chemical mechanism, the FIK would allow for in vivo cell imaging, essentially providing continuous observation of cell proliferation with minimal interference from cell metabolism. Fluorescent microscopy could be done with live cells. 2) A single cell could be monitored through various stages of cell cycle. None of the existing methods allow for in vivo imaging of cell proliferation. Thus, conceptually, the difference between the capabilities of the existing assays and proposed FIK assay is analogous to the difference between a snapshot and a video recording. The broader impact/commercial potential of this project is to develop an assay for cell proliferation compatible with high-throughput screening technology. The assay can be applied to differentiate between quiescent and actively cycling stem cells, and, eventually, to differentiate between cancer stem cells and cancer cells. The low cost of reagents, simplicity of sample handling, and readiness to be adapted to a multi-well format make the proposed methodology an ideal candidate for application to high-throughput screening assays that will find applications in the fields of cancer biology and neurogenesis, as well as developmental and molecular biology. These fields all share a common research need for close observation of cell processes.

这个小型企业创新研究(SBIR)第一阶段的项目是将新的生物正交川津连接技术应用于DNA复制的荧光标记，并最终生产出用于细胞增殖的荧光成像试剂盒(FIK)。优点有两个：1)由于新的化学机制，FIK将允许在体内进行细胞成像，基本上提供对细胞增殖的连续观察，而对细胞新陈代谢的干扰最小。荧光显微镜可以用活细胞做。2)单个细胞可以通过细胞周期的不同阶段进行监测。现有的方法都不允许对细胞增殖进行活体成像。因此，从概念上讲，现有检测方法和拟议的FIK检测方法之间的能力差异类似于快照和视频记录之间的差异。该项目更广泛的影响/商业潜力是开发一种与高通量筛选技术兼容的细胞增殖检测方法。该方法可用于区分静止期和活跃期干细胞，并最终区分癌症干细胞和癌细胞。试剂成本低，样品处理简单，易于适应多孔格式，这使得所提出的方法成为应用于高通量筛查分析的理想候选者，这些分析将在癌症生物学和神经发生以及发育和分子生物学领域得到应用。这些领域都有一个共同的研究需求，即密切观察细胞过程。