Collaborative Research: The Contribution of Polyamines to N and C cycling in Marine Systems

合作研究：多胺对海洋系统中 N 和 C 循环的贡献

• 批准号: 1029742
• 负责人: James Hollibaugh
• 金额: $ 18.99万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1029742&HistoricalAwards=false
• 关键词: Collaborative; Research; Contribution; Polyamines; cycling

While dissolved organic nitrogen (DON) plays a central role in global ocean N cycling, little is known about specific DON components. To address one aspect of this, investigators from Kent State University and the University of Georgia will examine polyamines, an under-studied microbially-produced DON component. They hypothehesize that, despite the low concentrations of these N-rich compounds, free polyamines have an important role in microbial ecology. To test this hypothesis, the investigators will examine the concentration, flux and fate of dissolved polyamines and dissolved amino acids at nearshore and open ocean sites using various chemical analyses. The composition of bacterial assemblages and of genes involved in polyamine utilization will also be determined using state-of-the-art molecular techniques. By integrating biogeochemical and molecular approaches, and linking functional genomicsof plankton assemblages to material fluxes, the work will improve the understanding of the fate and dynamics of DON in marine environments, and the importance of polyamines as sources of C and N. This collaborative project willenhance inter-institution partnerships, and train graduate students while alsoreaching out to under-represented and promising first- andsecond-year undergraduate and high school students. The students will be involved with research opportunities, summer internships, and other activities.

虽然溶解有机氮（DON）在全球海洋氮循环中起着核心作用，但对具体的DON组分知之甚少。为了解决这个问题的一个方面，来自肯特州立大学和格鲁吉亚大学的研究人员将研究多胺，一种研究不足的微生物产生的DON成分。他们假设，尽管这些富氮化合物的浓度很低，但游离多胺在微生物生态学中具有重要作用。为了验证这一假设，研究人员将使用各种化学分析来检查近岸和公海站点溶解的多胺和溶解的氨基酸的浓度，通量和命运。还将使用最先进的分子技术来确定细菌组合物和多胺利用所涉及的基因的组成。通过整合生物地球化学和分子方法，并将浮游生物组合的功能基因组学与物质通量联系起来，这项工作将提高对DON在海洋环境中的命运和动态以及多胺作为C和N来源的重要性的理解。这一合作项目将加强机构间的伙伴关系，培养研究生，同时也接触到代表性不足和有前途的一年级和二年级本科生和高中生。学生将参与研究机会，暑期实习和其他活动。