Structure and function of ribosome biogenesis factors and their functional complexes with small ribosomal subunit RNAs and RNPs (B10)

真核生物的核糖体组装是一个高度复杂的过程，需要约200个核糖体组装因子的协同作用。利用它们保守的古细菌同源物，可以获得它们在核糖体组装中的重要结构和功能见解。我们将研究核糖体组装因子、核糖体蛋白和rrna元件之间的功能相互作用的结构基础，这些元件参与小核糖体亚基生物发生的后期阶段，重点是核糖体蛋白-伴侣系统。我们将直接比较古细菌和真核生物系统，以深入了解核糖体组装途径的演变。

Ribosome assembly in eukaryotes is a highly complex process that requires the concerted action of ~ 200 ribosome assembly factors. Important structural and functional insights into their role in ribosome assembly can be obtained using their conserved archaeal homologs. We will investigate the structural basis for the functional interactions between ribosome assembly factors, ribosomal proteins and rRNA-elements involved in the late stages of small ribosomal subunit biogenesis with a focus on ribosomal proteins-chaperone systems. We will directly compare archaeal and eukaryotic systems to gain insight into the evolution of the ribosome assembly pathway.