MRI: Acquisition of a Multifrequency Phase and Modulation Fluorimeter

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This Major Research Instrumentation award funds the acquisition of a multifrequency cross-correlation phase and modulation fluorimeter at the University of Kentucky Center for Structural Biology. The fluorimeter is used to study the structure, stability and interactions of proteins and other biological macromolecules. Major experiments that will be performed with the fluorimeter include fluorescence resonance energy transfer, providing information on the distribution of distances between fluorophores attached to biomolecules, and anisotropy (polarization), to study interactions between biomolecules. Some of the molecules of interest in these studies include neurolysin, neuropeptidase, and nitroreductase. In addition, the instrument is available for use by investigators at other regional institutions and used in the annual Biofluorescence Symposium at the University of Kentucky. The results of these research and teaching efforts will be broadly disseminated through abstracts and peer reviewed publications, as well as by active participation of students and faculty at professional meetings.