MRI: Acquisition of an RT-PCR System for Research in Cell Biology, Physiology, Protein Biochemistry, Supramolecular Chemistry and Anthropology at New Paltz College

该主要研究仪器奖资助了真实的时间PCR热循环仪系统的收购。该设备基本上由用于聚合酶链式反应（PCR）的常规热循环仪和荧光检测系统组成。该系统的一个常见应用称为真实的时间PCR（RT-PCR），因为当样品经历PCR扩增时可以监测荧光。将使用RT-PCR系统的实验包括依赖于核酸的PCR扩增的常规分子细胞生物学测定，以及蛋白质生物化学和非基于PCR的超分子合成化学中的相对新颖的应用。 RT-PCR系统将用于通过量化基因表达水平更好地了解酵母中的细胞分裂;探索与昆虫进食有关的不同胰岛素样蛋白质的基因表达模式;实验性地记录X射线照射对考古样品的影响;开发用于鉴定新的核激素受体配体的测定，并有助于筛选设计用作荧光分子传感器的一系列新型化学品。 本提案中描述的所有项目都将在本科生的协助下进行。使用新的热循环仪进行的研究结果将通过摘要和同行评审的出版物以及学生和教师积极参与专业会议来广泛传播。

This Major Research Instrumentation award funds the acquisition of a Real Time PCR thermocycler system. This piece of equipment essentially consists of a conventional thermal cycler used for polymerase chain reaction (PCR) and a fluorescence detection system. A common application for this system is called Real Time PCR (RT-PCR) because fluorescence can be monitored as samples undergo PCR amplification. The experiments that will use the RT-PCR system include the conventional Molecular Cell Biology assays that rely on PCR amplification of nucleic acids, as well as relatively novel applications in protein biochemistry and supramolecular synthetic chemistry that are not PCR-based. The RT-PCR system will be used to better understand cell division in yeast by quantifying gene expression levels; explore gene expression patterns of different insulin-like proteins involved in insect feeding; experimentally document the effect of X-irradiation on archaeological samples; develop an assay to be used to identify novel nuclear hormone receptor ligands, and facilitate screening of a range of novel chemicals designed to serve as fluorescent molecular sensors. All of the projects described in this proposal will be conducted with the assistance of undergraduate students. Results from the studies conducted with the new thermocycler will be broadly disseminated through abstracts and peer reviewed publications, as well as by active participation of students and faculty at professional meetings.