From phalanx to assassins: The commensal-to-pathogen switch in the lepidopteran gut microbial community

从方阵到刺客：鳞翅目肠道微生物群落中的共生菌到病原体的转变

• 批准号: 1041557
• 负责人: Jo Handelsman
• 金额: $ 17.73万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1041557&HistoricalAwards=false
• 关键词: phalanx; assassins; commensal; pathogen; switch

In many animals, bacteria vastly outnumber of animal cells. Most of the associations between animals and bacteria are benign and some are beneficial, but under some circumstances, commensal (benign) bacteria convert to pathogens, resulting in diseases such as endocarditis and sepsis. This switch is poorly understood, but may involve changes in host physiology or immune status or bacterial entry into organs where they normally do not reside. The animal gut is a common source of bacteria that undergo the commensal-to-pathogen switch, but the complexity of the microbial communities in many animals' guts presents a barrier to studying the gut community's interactions with the host that lead to the change in health status. The investigators will study the commensal-to-pathogen switch of normal gut residents in a common caterpillar, the larva of the gypsy moth. The project aims to understand the fundamental ecological interactions that enable a bacterium that is a normal member of the gypsy moth gut flora to incite disease and death in the presence of a toxin produced by another bacterium. The study will test the hypotheses that the conversion to pathogen is associated with host's inflammatory response and that the normal microbial community of the gut mediates the commensal-to-pathogen switch. The project will provide the context for providing summer research experiences for undergraduate students from historically black universities. They will be recruited from participating universities in cohorts to provide them a peer group during the research program. All mentors of the summer students will be trained in the course, Entering Mentoring, which was developed by the PI, to ensure a successful research experience for every student.

在许多动物体内，细菌的数量远远超过动物细胞。动物和细菌之间的联系大多数是良性的，有些是有益的，但在某些情况下，共生（良性）细菌转化为病原体，导致心内膜炎和败血症等疾病。人们对这种转换知之甚少，但可能涉及宿主生理或免疫状态的变化，或者细菌进入它们通常不存在的器官。动物肠道是经历共生到病原体转换的细菌的常见来源，但许多动物肠道中微生物群落的复杂性为研究肠道群落与宿主相互作用导致健康状况变化的障碍。研究人员将研究一种常见的毛虫，舞毒蛾的幼虫，正常肠道居民的评论到病原体的转换。该项目旨在了解基本的生态相互作用，这种相互作用使舞毒蛾肠道菌群中的正常细菌在另一种细菌产生的毒素存在下引发疾病和死亡。该研究将验证以下假设：向病原体的转化与宿主的炎症反应有关，肠道的正常微生物群落介导了共生菌向病原体的转换。该项目将为来自历史悠久的黑人大学的本科生提供暑期研究经验。他们将从参与研究的大学中被招募出来，在研究项目期间为他们提供一个同伴小组。所有暑期学生的导师都将接受由PI开发的“进入指导”课程的培训，以确保每个学生都能获得成功的研究经验。