From phalanx to assassins: The commensal-to-pathogen switch in the lepidopteran gut microbial community

在许多动物中，细菌的数量远远超过动物细胞。 动物和细菌之间的大多数联系是良性的，有些是有益的，但在某些情况下，肠道（良性）细菌转化为病原体，导致心内膜炎和败血症等疾病。 这种转换还不太清楚，但可能涉及宿主生理学或免疫状态的变化，或细菌进入它们通常不存在的器官。 动物肠道是细菌的常见来源，这些细菌经历了细菌到病原体的转换，但许多动物肠道中微生物群落的复杂性阻碍了研究肠道群落与宿主的相互作用，导致健康状况的变化。 研究人员将研究一种常见的毛虫（舞毒蛾的幼虫）的正常肠道居民的肠道到病原体的转换。 该项目的目的是了解基本的生态相互作用，使一种细菌，这是一个正常的成员吉普赛蛾肠道植物群煽动疾病和死亡的毒素存在的另一种细菌。 该研究将测试以下假设：向病原体的转化与宿主的炎症反应有关，肠道的正常微生物群落介导了肠道向病原体的转换。 该项目将为来自历史悠久的黑人大学的本科生提供夏季研究经验提供背景。 他们将从参与大学中招募，在研究计划期间为他们提供一个同龄人小组。 暑期学生的所有导师将在课程中接受培训，进入导师，这是由PI开发，以确保每个学生的成功研究经验。

In many animals, bacteria vastly outnumber of animal cells. Most of the associations between animals and bacteria are benign and some are beneficial, but under some circumstances, commensal (benign) bacteria convert to pathogens, resulting in diseases such as endocarditis and sepsis. This switch is poorly understood, but may involve changes in host physiology or immune status or bacterial entry into organs where they normally do not reside. The animal gut is a common source of bacteria that undergo the commensal-to-pathogen switch, but the complexity of the microbial communities in many animals' guts presents a barrier to studying the gut community's interactions with the host that lead to the change in health status. The investigators will study the commensal-to-pathogen switch of normal gut residents in a common caterpillar, the larva of the gypsy moth. The project aims to understand the fundamental ecological interactions that enable a bacterium that is a normal member of the gypsy moth gut flora to incite disease and death in the presence of a toxin produced by another bacterium. The study will test the hypotheses that the conversion to pathogen is associated with host's inflammatory response and that the normal microbial community of the gut mediates the commensal-to-pathogen switch. The project will provide the context for providing summer research experiences for undergraduate students from historically black universities. They will be recruited from participating universities in cohorts to provide them a peer group during the research program. All mentors of the summer students will be trained in the course, Entering Mentoring, which was developed by the PI, to ensure a successful research experience for every student.