Rho-GTPase-dependent plasmacytoid dendritic cell seeding, retention and recruitment in vivo (A06)

我们提出了一种新的PDC迁移方案：以外周组织为PDC储存库，在稳定状态下从血液向外周组织播种，并通过适当的刺激向炎症部位募集。第一个结果表明，pDC受Rho家族GTPase CDc42控制，因为CDc42-Ko pDCs的募集功能受损。在pDC重新进入循环之前，将特别关注pDC的动力学、路径和与其他细胞的相互作用序列。对PDC周转、增殖能力和归巢的分子机制的分析将揭示CDC42参与PDC的发育、功能和分布。

We propose a new scheme of pDC-migration: seeding from blood to peripheral tissues in steady-state and recruitment to site of inflammation by appropriate stimuli with peripheral tissues as pDC-reservoirs. First results indicate that pDCs are controlled by the Rho-family GTPase Cdc42, as Cdc42-ko pDCs display impaired recruitment. A specific focus will be given to the kinetic, route and interac-tion sequences of pDCs with other cells before they re-renter the circulation. Analyses of molecular mechanisms of turnover, proliferative capacity and homing will reveal the involvement of Cdc42 in pDC development, function and distribution.