Role of thrombospondins for leukocyte trafficking to inflamed tissue (B03)

血小板反应蛋白在白细胞运输至发炎组织中的作用 (B03)

• 批准号: 200805686
• 金额: --
• 依托单位: Institut für Chirurgische Forschung
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200805686?language=en
• 关键词: Role; thrombospondins; leukocyte; trafficking; inflamed

In this project, we will elucidate the role of thrombospondins (TSPs) for myeloid leukocyte trafficking to inflamed tissue. To this end, we will first define the interactome of these matricellular proteins by mass spectrometry. Subsequently, we will employ advanced in vitro/ex vivo assays and in vivo microscopy techniques to characterize the effect of TSPs on intravascular interactions as well as on the extravascular locomotion of neutrophils and monocytes. Finally, we will use genetically altered mice to evaluate the functional relevance of TSPs for the trafficking of these immune cells in experimental sepsis.

在这个项目中，我们将阐明血栓反应蛋白（tsp）在骨髓白细胞运输到炎症组织中的作用。为此，我们将首先用质谱法确定这些基质细胞蛋白的相互作用。随后，我们将采用先进的体外/离体分析和体内显微镜技术来表征tsp对血管内相互作用以及中性粒细胞和单核细胞血管外运动的影响。最后，我们将使用转基因小鼠来评估tsp与实验性败血症中这些免疫细胞运输的功能相关性。