Develop Conditionaly Charged Residues for the Design of Peptide Channels

开发用于肽通道设计的条件带电残基

• 批准号: 1112188
• 负责人: Jianmin Gao
• 金额: $ 33万
• 依托单位: Boston College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1112188&HistoricalAwards=false
• 关键词: Develop; Conditionaly; Charged; Residues; Design

With this award, the Chemistry of Life Processes program is supporting the research of Professor Jianmin Gao of the Department of Chemistry at Boston College. Professor Gao will develop and test a novel class of unnatural amino acids that change their ionization states upon interacting with lipid membranes. These amino acids are designated as "Conditionally Charged Residues" (CCRs) as they are designed to be positively charged in aqueous solution, but neutral when placed in a nonpolar environment such as a lipid membrane. It is expected that such CCRs will have broad application in bioorganic and medicinal chemistry. In this project, the synthesized CCRs are to be incorporated into the well characterized channel forming peptide, gramicidin A, as a model system. Professor Gao will examine the structural determinants of peptides that enable their efficient folding into transmembrane channels. The Broader Impacts of this project include the development of a set of widely applicable non-proteinogenic amino acids for use in chemical biology and their deployment toward advancing our understanding of peptide-membrane interactions. Of equal importance is the education and training of graduate and undergraduate students in interdisciplinary research. The training program includes an outreach component through the intern program that the PI has initiated with Roxbury Community College (RCC).

通过这个奖项，生命过程化学项目支持了波士顿学院化学系高建民教授的研究。高教授将开发和测试一类新型的非天然氨基酸，这种氨基酸在与脂质膜相互作用时改变其电离状态。这些氨基酸被称为“条件带电残基”（ccr），因为它们在水溶液中被设计为带正电，但在非极性环境（如脂质膜）中被设计为中性。在生物有机化学和药物化学领域具有广阔的应用前景。在本项目中，合成的CCRs将被纳入表征良好的通道形成肽gramicidin A中作为模型系统。高教授将研究肽的结构决定因素，使它们能够有效地折叠成跨膜通道。该项目的更广泛影响包括开发一套广泛应用于化学生物学的非蛋白质原性氨基酸，以及它们的部署，以推进我们对肽-膜相互作用的理解。对研究生和本科生进行跨学科研究的教育和培训也同样重要。该培训计划包括通过PI与罗克斯伯里社区学院（RCC）发起的实习生计划的外展部分。