Collaborative Research: Advancing In-vivo Chemical Imaging by Merging Synchrotron-Based Infrared Data, Rapid Spectral Image Analysis, and Quantitative Chemometrics

威斯康星大学密尔沃基分校的Carol J.Hirschmugl教授和Roshan M Dourza教授以及田纳西诺克斯维尔大学的Frank Vogt教授得到了化学系化学测量和成像(CMI)计划的支持，以开展一个促进体内化学成像和分析的合作项目。这项研究将通过使用威斯康星州麦迪逊同步辐射中心的高光谱成像光谱仪(IRENI)获得的基于同步加速器的红外数据与使用图形处理单元和定量化学计量学的快速光谱图像分析相结合来进行。这项研究涉及活细胞的高分辨率成像、散射校正、复杂系统中浓度测量的化学计量学方法以及在藻类生长研究中的应用。如果成功，该项目将改善IRENI的性能，这是同类设备中的高级设备，并使以前被认为过于复杂而无法处理的样本，如活细胞的化学成像和分析成为可能。该项目将在化学和生物领域产生广泛影响。学生们将在同步辐射中心接受最先进的红外成像仪器的培训。来自不同群体的本科生和他们的老师将通过现有的REU和RET方案参与该项目，并将举办讲习班，使社区熟悉IRENI并扩大其技术用户基础。

Professors Carol J. Hirschmugl and Roshan M Dsouza of the University of Wisconsin-Milwaukee and Professor Frank Vogt of the University of Tennessee Knoxville are supported by the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry to conduct a collaborative project to advance in-vivo chemical imaging and analysis. The research will be conducted by combining synchrotron-based infrared data obtained with the hyperspectral imaging spectrometer (IRENI) at the Synchrotron Radiation Center at Madison, WI with rapid spectral image analysis using graphics processing units and quantitative chemometrics. The research involves high resolution imaging of live cells, scattering corrections, chemometric methods for concentration measurement in complex systems and application to algae growth studies. If successful the project will improve the performance of IRENI, the premium facility of its kind, and enable the chemical imaging and analysis of samples that were previously considered to be too complex to handle, such as live cells. The project will have a broad impact in chemistry and biology. Students will be trained on a state-of-the-art infrared imaging instrument in a Synchrotron Radiation Center. Undergraduate students from diverse groups and their teachers will be involved in the project through existing REU and RET programs, and workshops will be conducted to familiarize the community with IRENI and to broaden its technical user base.