RIG: Kinetic Characterization of F420 Dependent Glucose- 6-Phosphate Dehydrogenase from M. tuberculosis

RIG：结核分枝杆菌 F420 依赖性葡萄糖-6-磷酸脱氢酶的动力学表征

• 批准号: 1120837
• 负责人: Kayunta Johnson-Winters
• 金额: $ 20万
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1120837&HistoricalAwards=false
• 关键词: RIG; Kinetic; Characterization; F420; Dependent

F420 Cofactor Dependent Glucose-6-phosphate Dehydrogenase (FGD) catalyzes the conversion of Glucose-6-phosphate to 6-phosphogluconolactone. The enzymology of F420 dependent enzymes, such as FGD, has not yet been subject to rigorous investigation. The overall goal of this research is to understand the function and hydride transfer reactivity of FGD from Mycobacterium tuberculosis using site-directed mutagenesis, kinetic isotope effects (KIEs), steady-state and pre-steady state methods. Broader Impacts: This project will support the training of minority students in biochemistry. This work will also enhance the quality of education for participating students because they will acquire essential biochemical skills in the field of enzymology. As an African-American female, the PI is aware of the scarcity of under-represented groups in the scientific community and would like to provide mentoring opportunities to all students interested, including those from under-represented groups.

F420辅因子依赖的葡萄糖-6-磷酸脱氢酶(FGD)催化葡萄糖-6-磷酸转化为6-磷酸葡萄糖内酯。F420依赖酶的酶学，如FGD，还没有受到严格的研究。本研究的总体目标是利用定点突变、动力学同位素效应(Kies)、稳态和稳态前的方法来了解结核分枝杆菌FGD的功能和氢化物转移反应。更广泛的影响：该项目将支持少数民族学生在生物化学方面的培训。这项工作还将提高参与学生的教育质量，因为他们将获得酶学领域的基本生化技能。作为一名非裔美国女性，国际学生联合会意识到科学界代表性不足的群体很少，并愿意为所有感兴趣的学生提供指导机会，包括来自代表性不足群体的学生。