Genetic Control of Vulval and Uterine Coordination in C. elegans: egl-26 and vulF

线虫外阴和子宫协调的遗传控制：egl-26 和 vulF

• 批准号: 1121386
• 负责人: Wendy Hanna-Rose
• 金额: $ 40.2万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1121386&HistoricalAwards=false
• 关键词: Genetic; Control; Vulval; Uterine; Coordination

Many if not most human organs have ducts or tubes that contribute to organ function. The molecular and genetic mechanisms that direct cells to form the walls of tubes surrounding open lumens are not fully elucidated. This project aims to address the question "How do cells form tubes?" There isn't just one answer to this question because the cellular rearrangements contributing to tube formation in different organs and even within different areas of a single biological tube are varied. However, it is important to understand multiple specific examples of tube formation because failure of these biological programs can result in birth defects (for example, lack of connection between two tubes such as the esophagus and the stomach), and excess activity of these programs can contribute to disease (for example, blood vessel formation promotes tumor growth). This project will focus on understanding tube formation in the reproductive system in the microscopic nematode C elegans. Genetic approaches will allow discovery of new genes affecting tubulogenesis. By using microscopy on live developing tissues, the specific changes in cell structure that accompany tube formation will also be determined. Many biological mechanisms first revealed in C. elegans have proven relevant to all metazoans. Thus, the project has broad intellectual merit because tubulogenesis principles elucidated in C. elegans will have corollaries in other systems and, thus, wide-reaching significance to our understanding of organ formation. A core part of this project is a formal course with an integrated research component that will serve to recruit and train undergraduate students to pursue advanced degrees in biology. Similarly, graduate students will be trained in preparation for independent science careers. Thus, the project has broad merit in the educational realm as well. An important outcome from the completed study will be the preparation of the next generation of scientists.

许多（如果不是大多数）人体器官都有有助于器官功能的导管或管道。指导细胞形成开放腔周围管壁的分子和遗传机制尚未完全阐明。本项目旨在解决“细胞如何形成管？“这个问题的答案并不只有一个，因为在不同的器官中，甚至在单个生物管的不同区域内，有助于管形成的细胞重排是不同的。然而，重要的是要了解管道形成的多个具体例子，因为这些生物程序的失败可能导致出生缺陷（例如，两个管道之间缺乏连接，如食道和胃），这些程序的过度活动可能导致疾病（例如，血管形成促进肿瘤生长）。本计画将著重于了解线虫生殖系统中管状结构的形成。遗传学方法将允许发现影响小管发生的新基因。通过在活的发育组织上使用显微镜，还将确定伴随管形成的细胞结构的具体变化。许多生物学机制首先在C.已经证明秀丽线虫与所有后生动物都有关系。因此，该项目具有广泛的知识价值，因为C.在其他系统中也会有推论，因此，对我们理解器官的形成具有广泛的意义。这个项目的核心部分是一个正式的课程，与综合研究组成部分，将有助于招募和培训本科生攻读生物学高级学位。 同样，研究生将接受培训，为独立的科学事业做准备。 因此，该项目在教育领域也具有广泛的价值。完成研究的一个重要成果将是培养下一代科学家。