Compositional and functional analysis of ribosomal RNA gene chromatin in distinct transcriptional states (A05)

不同转录状态下核糖体RNA基因染色质的组成和功能分析（A05）

• 批准号: 202824917
• 金额: --
• 依托单位: Lehrstuhl für Biochemie III
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/202824917?language=en
• 关键词: Compositional; functional; analysis; ribosomal; RNA

We developed a new strategy allowing the in vivo analysis and purification of RNA polymerase (Pol) I transcribed episomal chromatin. Episomal chromatin will be analyzed using established and complementary ex vivo and in vivo approaches. We propose to pursue two main research goals:(i) to analyse structure function relationships within the RNA polymerase (Pol) I preinitiation complex (PIC) and to determine the molecular requirements for its assembly(ii) to define the molecular nature of the open Pol I transcribed chromatin state, its contribution to Pol I transcription, and the mechanisms required for its establishment.We expect high synergy in particular with project AP1 in a comparative functional and structural analysis of Pol I PICs obtained by in vitro reconstitution or after in vivo assembly in yeast. We will further continue our collaboration with project A1 in the reconstitution of Pol I transcription from native chromatin templates in vitro. We suppose that this work will contribute to our understanding of the intimate interrelationship between chromatin structure and the process of nuclear transcription in eukaryotes.

我们开发了一种新的策略，允许在体内分析和纯化的RNA聚合酶（Pol）I转录的附加体染色质。将使用已建立的和互补的离体和体内方法分析附加体染色质。我们建议追求两个主要的研究目标：（i）分析结构功能的关系内的RNA聚合酶（Pol）I preinitiation复合物（PIC），并确定其组装的分子要求（ii）定义的分子性质的开放Pol I转录染色质状态，其贡献Pol I转录，并为它的建立所需的机制，我们期望高协同作用，特别是与项目AP 1在一个比较功能和结构分析Pol I PIC通过体外重组或在酵母体内组装后获得。我们将进一步继续与A1项目合作，在体外从天然染色质模板重建Pol I转录。我们认为，这项工作将有助于我们了解染色质结构和核转录过程之间的密切关系在真核生物。