Identification and Characterization of Functional Constraints Operating on the Gene Organization of the Drosophila Genome

该项目将促进对染色体上基因组织与改变染色体的大突变发生概率之间关系的理解。染色体上的基因组织对于基因的正常功能是重要的，这有时需要染色体上遥远的遗传因子的相互作用。能够操纵果蝇染色体的方法将被用来测试在这些长期效应的影响下，诱导重排对基因功能的影响。该项目旨在回答为什么染色体的特定区域在远亲物种中保守，而其他区域往往表现出与大突变不同寻常的关联。更广泛的影响：从培养的角度来看，本项目将帮助培养1名博士后、6名本科生和6名高中生进行基因组功能和进化生物学的分析。代表人数不足的群体的成员将优先受益于将要开展的研究活动。参加者也将有机会磨练他们的演讲技巧。此外，该项目的具体活动将被改编并融入PI的教学活动中。产生的所有数据和材料将向科学界公开提供，并存放在适当的存储库中。

This project will advance understanding of the relationship between the organization of genes on chromosomes and the probability of occurrence of macromutations that alter chromosomes. The organization of genes on chromosomes can be important for proper gene function, which sometimes requires the interplay of genetic factors far apart on the chromosome. Methods that enable the manipulation of fruit flies chromosomes will be used to test the consequences of induced rearrangements on the function of genes putatively under the influence of these long-range effects. This project aims at answering why particular regions of chromosomes have been conserved among distantly related species while others often exhibit an unusual association with macromutations.Broader Impacts: From the training standpoint, this project will help train one postdoc, six undergraduates, and six high-school students in the analysis of the function of genomes and evolutionary biology. Members of underrepresented groups will be preferentially benefited by the research activities to be developed. Participants will also have the opportunity to hone their presentation skills. In addition, specific activities in this project will be adapted and integrated into the teaching activities of the PI. All data and materials generated will be made publicly available to the scientific community and deposited in appropriate repositories.