EAGER: Impact of nanoparticles containing rare earth elements onto different bacterial communities

1203555-由于稀土元素在工业和军事上的重要应用，如生物医学仪器、绿色能源产品和生物技术等，稀土元素目前受到广泛关注。 然而，关于其环境、健康或安全影响的信息很少，特别是作为纳米材料。 这项工作将开始发展的协议，以检查这些纳米材料可能产生的有害影响，并铺平了道路，这类nanomaterials.Intellectual MeritThis研究将（一）建立可靠的分析技术和实验方法，（二）评估稀土纳米粒子（nREE）的影响的大小，以不同的微生物种群，和（iii）产生初步的数据，为进一步的工作。 铈和镝纳米粒子将被表征和检查，就其对大肠杆菌的影响。大肠杆菌 二氧化铈的影响已得到充分研究，并将被用作对照，而镝纳米材料的影响尚不清楚。 将检查对细胞的影响，如活力、膜渗透、细胞生长、呼吸、细胞内区室化、不同培养基化学的影响。 这项研究开发的方法将提供有关这种稀土影响的新信息，以及检查同类其他材料的方法。拟议的研究将提供有关从这些纳米颗粒和纳米产品释放的nREE和REE离子的潜在毒性的基本数据。此外，还将评估这些材料在各种自然水条件下的命运。这些信息将有助于了解这些材料一旦释放到环境中所产生的影响。 研究生和本科生将从事这项研究，其中包括2名西班牙裔学生。这项工作将纳入方案研究所的课程，并通过科学和公共媒体传播成果。

1203555-CraverThere is much current interest in rare earth elements (REE) as a result of their critical uses in industrial and military applications in products such as biomedical instrumentation, green energy products, and . However, there is little information on their environment, health, or safety impacts, particularly as nanomaterials. This work will begin development of protocols to examine possible harmful effects of these nanomaterials and pave the way for a broader examination of this class of nanomaterials.Intellectual MeritThis research will (i) establish reliable analytical techniques and experimental methodologies, (ii) assess the magnitude of the impact of REE nanoparticles (nREE) to different populations of microorganisms, and (iii) generate preliminary data for further work. Cerium and Dysprosium nanoparticles will be characterized and examined with respect to their impact on E. coli bacteria. Ceria impacts have been well-studied and will be used as a control, while the effects of dysprosium nanomaterials are unknown. Effects on cells such as viability, membrane permeation, cell growth, respiration, compartmentalization within the cells, effects of different media chemistries will be examined. Methods developed in this research will provide both new information about the impact of this rare earth and the means of examining other materials in the same class. Broader Impacts The proposed study will provide fundamental data regarding the potential toxicity of nREEs and REE ions released from these nanoparticles and nanoproducts. Additionally,information regarding the fate of these materials in a variety of natural water conditions will be evaluated. This information will help to understand the impact of the materials once released into the environment. Graduate and undergraduate students will work on this research including 2 Hispanic students. The work will be incorporated into the PIs courses and the results disseminated through scientific and public media.