Mechanisms of Mitochondrial Deletions and Double-strand Break Repair

线粒体缺失和双链断裂修复机制

• 批准号: 1243428
• 负责人: Elaine Sia
• 金额: $ 75.28万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-01-01 至 2016-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1243428&HistoricalAwards=false
• 关键词: Mechanisms; Mitochondrial; Deletions; Double; strand

Intellectual merit: Eukaryotic cells contain mitochondria, the membrane-bound organelles that produce energy for the cell. Like the nucleus of the cell, mitochondria contain DNA genomes, whose maintenance without error is essential to the normal function of cells. Deletions of large segments of the mitochondrial DNA are common mutations in many types of cells. In many species, these mutations accumulate with age and are associated with metabolic syndromes. Deletions of this type are also commonly observed in the mitochondrial DNA of baker's yeast. The molecular tools available for research in yeast have made it a powerful model for the study of mitochondrial biology. The long-term goal of this project is to understand the fundamental cellular processes that contribute to mitochondrial deletion mutations. Studies are directed at understanding the proteins involved in this process and their activities, as well as understanding the role of double-strand breaks in the generation of deletion mutations. Completion of this work will provide information on the broad area of mitochondrial DNA transactions, including replication, recombination and repair.Broader impacts: This work will have a positive impact on the education of undergraduates in the biological sciences at two institutions, as this project represents a collaborative effort between labs at two local universities. Dr. Elaine Sia, at the University of Rochester, and Dr. Rey Sia, at SUNY-Brockport have a history of undergraduate mentorship of undergraduates in the laboratory, with a combined record of advising 20 undergraduates in independent study in the past 2.5 years. Studies with yeast are well suited to undergraduates with little experience, allowing them to learn a large number of techniques and concepts in a summer or semester. Special effort will continue to be made to recruit individuals from groups underrepresented in science, or first generation college students via connections with CSTEP/McNair, and summer REU programs. The PI and co-PI continue to honor their commitment to scientific education with the involvement of undergraduate biology majors at SUNY Brockport and the University of Rochester in this project.

智力优点：真核细胞含有线粒体，线粒体是为细胞产生能量的膜结合细胞器。 像细胞核一样，线粒体包含DNA基因组，其无错误的维护对细胞的正常功能至关重要。 线粒体DNA的大片段缺失是许多类型细胞中常见的突变。 在许多物种中，这些突变随着年龄的增长而积累，并与代谢综合征有关。 这种类型的缺失也通常在面包酵母的线粒体DNA中观察到。 可用于酵母研究的分子工具使其成为研究线粒体生物学的强大模型。 该项目的长期目标是了解导致线粒体缺失突变的基本细胞过程。 研究旨在了解参与这一过程的蛋白质及其活性，以及了解双链断裂在产生缺失突变中的作用。 这项工作的完成将为线粒体DNA的复制、重组和修复等广泛领域提供信息。更广泛的影响：这项工作将对两个机构的生物科学本科生教育产生积极影响，因为该项目代表了两所当地大学实验室之间的合作努力。 罗切斯特大学的伊莱恩·西亚博士和纽约州立大学布罗克波特分校的雷伊·西亚博士有着在实验室指导本科生的历史，在过去的2.5年里，他们总共指导了20名本科生进行独立学习。 酵母研究非常适合经验不足的本科生，使他们能够在一个夏天或一个学期内学习大量的技术和概念。 将继续特别努力，通过与CSTEP/McNair和夏季REU计划的联系，从科学代表性不足的群体或第一代大学生中招募个人。 PI和co-PI继续荣誉他们对科学教育的承诺，纽约州立大学布罗克波特分校和罗切斯特大学的本科生物学专业的学生参与了这个项目。