Collaborative Research: Completing single- and cross-hole hydrogeologic and microbial experiments: Juan de Fuca Flank

2010年，IODP exp. 327号发射了一系列实验，这些实验基于在胡安·德富卡山脊（JDFR）上和附近的6个钻孔（1026 B，1027 C，1301 A，1301 B，1362 A和1362 B孔）中放置的仪器。这些实验旨在研究玄武岩洋壳的水文和生物过程。要解决的主要问题包括整体渗透性，流体，溶质和热传输的大小，速度和方向，对地壳的流体存储特性的约束，确定流体储层如何响应地震扰动，并调查的变异性，多样性和代谢的居民微生物种群。该项目将在装有仪器的钻孔中回收传感器和采样器，获取数据和样本，收集放置在钻孔深处的生物培养器，并密封装有仪器的钻孔。所有这些活动都是完成JDFR实验所必需的。一旦数据和样本被取回，PI将根据流动测试的结果，为海洋地壳的水文学（渗透性、渗透区的厚度和范围、连通性、流动方向和各向异性）开发新的数值模型。T传感器将使热结构与流动参数一起确定。DNA将通过？16S核糖体RNA（rRNA）基因以及选择全长桑格式测序和基因指纹技术。PI将关注多样性，共享物种，群体的出现和消失。更广泛的影响包括培训4名船上教育工作者，重点是为这些职位招募代表性不足的群体，教师研讨会，以及对本科生和研究生以及博士后的特殊培训。研究团队包括研究生、本科生和博士后。

In 2010, IODP exp. 327 launched series of experiments based on instrumentation placed in 6 boreholes (Holes 1026B, 1027C, 1301A, 1301B, 1362A, and 1362B) on and near the Juan de Fuca Ridge (JDFR). The experiments were designed to examine hydrological and biological processes in basaltic ocean crust. Major issues to be addressed include overall permeability, the magnitudes, velocity and directions of fluid, solute,and heat transport,constraints on the fluid storage properties of crust, determining how fluid reservoirs respond to seismic perturbations, and investigating the variability, diversity, and metabolism of resident microbial populations. This project will recover sensors and samplers in the instrumented boreholes(CORKs),access data and samples, collect biological incubators placed at depth within the boreholes, and seal the CORKs. All of these activities are needed for the completion of the JDFR experimentsOnce the data and samples are retrieved, the PI's will develop new numerical models for the hydrology (permeability, thickness and extent of permeable zones, connectivity, direction of flow, and anisotropy) of ocean crust based on the results of flow tests. T sensors will enable thermal structure to be determined in conjunction with flow parameters. DNA will be analyzed via ?16S ribosomal RNA (rRNA) genes as well as select full-length Sanger-style sequencing and gene fingerprinting techniques.? The PI's will look at diversity, shared species, emergence and disappearance of groups. Metagenomic work will be done on some samples.Broader Impacts include training of 4 shipboard educators, with an emphasis on recruiting underrepresented groups for these positions, teacher workshops, and special training for undergraduate and graduate students and post-docs. Research team includes grads, undergrads, and post-docs.