Collaborative Research: Completing single- and cross-hole hydrogeologic and microbial experiments: Juan de Fuca Flank

2010年，IODP Exp.327在胡安德富卡山脊(JDFR)及其附近的6个钻孔(1026B孔、1027C孔、1301A孔、1301B孔、1362A孔和1362B孔)中放置了一系列仪器，进行了一系列实验。这些实验旨在研究玄武岩洋壳中的水文和生物过程。需要解决的主要问题包括总体渗透率、流体、溶质和热传输的大小、速度和方向，对地壳流体存储特性的限制，确定流体储集层对地震扰动的响应，以及调查常驻微生物种群的变异性、多样性和新陈代谢。该项目将回收仪表孔(软木塞)中的传感器和取样器，获取数据和样本，收集放置在钻孔深处的生物孵化器，并密封软木塞。所有这些活动都是完成JDFR实验所必需的。一旦检索到数据和样本，PI将根据流动测试结果开发新的洋壳水文数值模型(渗透率、渗透层厚度和程度、连通性、流动方向和各向异性)。T传感器将使热结构能够与流动参数一起确定。DNA将通过16S核糖体RNA(RRNA)基因以及精选的桑格式全长测序和基因指纹技术进行分析。PI将着眼于多样性、共享物种、群体的出现和消失。将在一些样本上进行元基因组学工作。广泛的影响包括培训4名船上教育工作者，重点是招募代表不足的群体担任这些职位，教师研讨会，以及对本科生、研究生和博士后的特殊培训。研究团队包括毕业生、本科生和博士后。

In 2010, IODP exp. 327 launched series of experiments based on instrumentation placed in 6 boreholes (Holes 1026B, 1027C, 1301A, 1301B, 1362A, and 1362B) on and near the Juan de Fuca Ridge (JDFR). The experiments were designed to examine hydrological and biological processes in basaltic ocean crust. Major issues to be addressed include overall permeability, the magnitudes, velocity and directions of fluid, solute,and heat transport,constraints on the fluid storage properties of crust, determining how fluid reservoirs respond to seismic perturbations, and investigating the variability, diversity, and metabolism of resident microbial populations. This project will recover sensors and samplers in the instrumented boreholes(CORKs),access data and samples, collect biological incubators placed at depth within the boreholes, and seal the CORKs. All of these activities are needed for the completion of the JDFR experimentsOnce the data and samples are retrieved, the PI's will develop new numerical models for the hydrology (permeability, thickness and extent of permeable zones, connectivity, direction of flow, and anisotropy) of ocean crust based on the results of flow tests. T sensors will enable thermal structure to be determined in conjunction with flow parameters. DNA will be analyzed via ?16S ribosomal RNA (rRNA) genes as well as select full-length Sanger-style sequencing and gene fingerprinting techniques.? The PI's will look at diversity, shared species, emergence and disappearance of groups. Metagenomic work will be done on some samples.Broader Impacts include training of 4 shipboard educators, with an emphasis on recruiting underrepresented groups for these positions, teacher workshops, and special training for undergraduate and graduate students and post-docs. Research team includes grads, undergrads, and post-docs.