Collaborative Research: Completing single- and cross-hole hydrogeologic and microbial experiments: Juan de Fuca Flank

2010年，IODP exp. 327在Juan de Fuca Ridge （JDFR）及其附近的6个井眼（1026B、1027C、1301A、1301B、1362A和1362B）中开展了一系列基于仪器的实验。这些实验旨在研究玄武岩海洋地壳的水文和生物过程。要解决的主要问题包括总体渗透率、流体的大小、速度和方向、溶质和热输运、地壳流体储存特性的限制、确定流体储层对地震扰动的反应，以及调查常驻微生物种群的变异性、多样性和代谢。该项目将回收仪器井眼（CORKs）中的传感器和采样器，获取数据和样本，收集放置在井眼深处的生物孵育器，并密封CORKs。所有这些活动都是完成JDFR实验所必需的。一旦数据和样品被检索出来，PI将根据流动测试的结果开发新的海洋地壳水文数值模型（渗透率、渗透带的厚度和范围、连通性、流动方向和各向异性）。T传感器将使热结构与流量参数一起确定。DNA将通过？16S核糖体RNA （rRNA）基因，以及选择全长桑格式测序和基因指纹技术。PI将关注多样性、共有物种、群体的出现和消失。将对一些样本进行宏基因组研究。更广泛的影响包括培训船上的教育工作者，重点是为这些职位招聘代表性不足的群体，教师研讨会，以及对本科生、研究生和博士后的特殊培训。研究团队包括研究生、本科生和博士后。

In 2010, IODP exp. 327 launched series of experiments based on instrumentation placed in 6 boreholes (Holes 1026B, 1027C, 1301A, 1301B, 1362A, and 1362B) on and near the Juan de Fuca Ridge (JDFR). The experiments were designed to examine hydrological and biological processes in basaltic ocean crust. Major issues to be addressed include overall permeability, the magnitudes, velocity and directions of fluid, solute,and heat transport,constraints on the fluid storage properties of crust, determining how fluid reservoirs respond to seismic perturbations, and investigating the variability, diversity, and metabolism of resident microbial populations. This project will recover sensors and samplers in the instrumented boreholes(CORKs),access data and samples, collect biological incubators placed at depth within the boreholes, and seal the CORKs. All of these activities are needed for the completion of the JDFR experimentsOnce the data and samples are retrieved, the PI's will develop new numerical models for the hydrology (permeability, thickness and extent of permeable zones, connectivity, direction of flow, and anisotropy) of ocean crust based on the results of flow tests. T sensors will enable thermal structure to be determined in conjunction with flow parameters. DNA will be analyzed via ?16S ribosomal RNA (rRNA) genes as well as select full-length Sanger-style sequencing and gene fingerprinting techniques.? The PI's will look at diversity, shared species, emergence and disappearance of groups. Metagenomic work will be done on some samples.Broader Impacts include training of 4 shipboard educators, with an emphasis on recruiting underrepresented groups for these positions, teacher workshops, and special training for undergraduate and graduate students and post-docs. Research team includes grads, undergrads, and post-docs.