DISSERTATION RESEARCH: Connecting mechanisms of variance in reproductive success to consequences of trait divergence among Tribolium species

论文研究：将繁殖成功率的差异机制与谷盗物种性状差异的后果联系起来

• 批准号: 1310878
• 负责人: Scott Pitnick
• 金额: $ 1.92万
• 依托单位: Syracuse University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1310878&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Connecting; mechanisms; variance

Traits that influence reproductive success can be both remarkably elaborate and differ greatly between related species. Variation in such traits not only influences an individual?s success in populations, but can also help form new species when groups differ in characteristics important to choosing a mate. Measuring reproductive success becomes more complex when animals mate with multiple individuals within a single breeding cycle, a common occurrence. Reproductive success, a major influence on trait evolution, is then shaped not only by success in achieving matings, but also by competitively fertilizing eggs, and producing high quality offspring. Using lines of flour beetles whose males? produce sperm with red or green fluorescent tagged heads, this research will evaluate the outcome of these different selective episodes and identify their contribution to reproductive success. The use of the fluorescent sperm lines also enables identification of mechanisms underlying differences in competitive fertilization success within populations, as well as differences in fertilization capability in hybrid versus same species matings. Finally, this research will explore how repeated matings influence offspring production in this highly promiscuous species.Because flour beetles are economically significant stored grain pests, insight to their reproductive system can inform management efforts. Additionally, the project will include involvement of multiple undergraduate researchers and outreach to the Syracuse City School District.

影响繁殖成功的特征可能非常复杂，并且在相关物种之间差异很大。这些特征的变化不仅会影响个体在种群中的成功，而且当群体在选择配偶时重要的特征不同时，还可以帮助形成新物种。当动物在一个繁殖周期内与多个个体交配时（这种情况很常见），衡量繁殖成功率变得更加复杂。繁殖成功是对性状进化的主要影响，它不仅取决于成功交配，还取决于竞争性的卵子受精和产生高质量的后代。使用哪些雄性的面粉甲虫品系？产生带有红色或绿色荧光标记头部的精子，这项研究将评估这些不同选择性事件的结果，并确定它们对生殖成功的贡献。荧光精子系的使用还能够识别种群内竞争性受精成功率差异的潜在机制，以及杂交与同种交配中受精能力的差异。最后，这项研究将探讨重复交配如何影响这种高度混杂的物种的后代生产。由于面粉甲虫是经济上具有重要意义的储粮害虫，因此了解其生殖系统可以为管理工作提供信息。此外，该项目将包括多名本科生研究人员的参与以及对锡拉丘兹市学区的推广。