DISSERTATION RESEARCH: Assessing evolution of euryhalinity in anchialine shrimps

论文研究：评估近海虾的广盐度演变

• 批准号: 1311500
• 负责人: Scott Santos
• 金额: $ 1.1万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1311500&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Assessing; evolution; euryhalinity

The molecular mechanisms of ionic/salt regulation in crustaceans have only been characterized for a narrow range of species, mainly crabs with a marine ancestry. Shrimp from coastal ponds and pools represent an opportunity to address this lack of knowledge since they have a decisively freshwater ancestry but have adapted to live in environments with drastically fluctuating salinities. Previous research found that Halocaridina rubra, a shrimp species endemic to Hawaii, has atypical patterns of gene expression following salinity transfer. To examine this in an evolutionary context, the molecular mechanisms of osmoregulation from additional shrimp species from the Ryukyus Islands, Japan will be investigated using high-throughput measures of gene expression (RNA-Seq). These shrimp species represent independent invasions of these habitats (from both freshwater and marine environments) and will further our limited understanding of the evolution of osmoregulation in crustaceans.Overall, this project will illuminate the evolution of osmoregulation, a critical biological process for all living organisms. Undergraduates from underrepresented groups will be trained and mentored during the project. Additionally, this system will be used to engage K-12 students in a variety of biological concepts.

甲壳类动物中离子/盐调节的分子机制仅在一小部分物种中得到表征，主要是具有海洋祖先的螃蟹。来自沿海池塘和水池的虾代表了解决这种知识缺乏的机会，因为它们具有决定性的淡水祖先，但已经适应了在盐度剧烈波动的环境中生活。先前的研究发现，夏威夷特有的一种虾种Halocaridina rubra在盐度转移后具有非典型的基因表达模式。为了在进化的背景下研究这一点，将使用高通量的基因表达（RNA-Seq）的措施，研究从琉球群岛，日本的其他虾种的ESTA调节的分子机制。这些虾种代表了这些栖息地的独立入侵（来自淡水和海洋环境），并将进一步加深我们对甲壳类动物中生物调节进化的有限理解。总体而言，该项目将阐明生物调节的进化，这是所有生物体的关键生物过程。在该项目期间，将对代表性不足群体的大学生进行培训和辅导。此外，该系统将用于使K-12学生参与各种生物概念。