SBIR Phase I: Smart Petri Dish with Built-in Imaging Capabilities for Cell Culture Monitoring

SBIR 第一阶段：具有内置成像功能的智能培养皿，用于细胞培养监测

• 批准号: 1315443
• 负责人: Phil Willems
• 金额: $ 14.58万
• 依托单位: ePetri Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1315443&HistoricalAwards=false
• 关键词: SBIR; Phase; Smart; Petri; Dish

This Small Business Innovation Research (SBIR) Phase I project will develop the Smart Petri Dish, a lens-free subpixel resolution microscope, which will automate cell culture monitoring from within the incubator in a low-cost, easy-to-use system that provides high-resolution images over a wide field of view. Current cell culture experiments require significant manual labor, making them costly and inconvenient. To develop the Smart Petri Dish, we will design a unique demountable image sensor cartridge with thermal control, develop a novel multithread sparse convolution filtering software algorithm for subpixel resolution image construction, enhance the spatial resolution of the lens-free imaging method with a dense illumination array, and test the resulting prototype Smart Petri Dish for its ability to reliably culture cells. It is anticipated that this research will demonstrate the feasibility of a commercially viable prototype system that can be further developed into a successful commercial product in Phase II of the project.The broader impact/commercial potential of this project, if successful, will be a system that will automate cell culture experiments and remove much of the cumbersome manual labor. As a low-cost, compact diagnostic tool, it will enable research and diagnosis in geographically dispersed regions where there is limited access to centralized cell culture laboratories. And, in primary education laboratories, it will introduce a large number of high school students to cell culture experimental methods and techniques, thanks to its low cost and simplicity of operation. Together, these impacts will enhance scientific and technological understanding of cell growth, and bring new tools to research, diagnosis, and education. The potential commercial value of the Smart Petri Dish is $10 M per year in revenues by the third year of production, based upon realistic projections of market size and potential market share.

这个小型企业创新研究（SBIR）第一阶段项目将开发智能培养皿，这是一种无透镜的亚像素分辨率显微镜，它将在一个低成本，易于使用的系统中从培养箱内自动进行细胞培养监测，该系统可在宽视场范围内提供高分辨率图像。目前的细胞培养实验需要大量的手工劳动，使得它们昂贵且不方便。为了开发智能培养皿，我们将设计一个独特的可拆卸的图像传感器墨盒与热控制，开发一种新的多线程稀疏卷积滤波软件算法的亚像素分辨率图像建设，提高空间分辨率的透镜无成像方法与密集照明阵列，并测试由此产生的原型智能培养皿的能力，可靠地培养细胞。预计该研究将证明商业上可行的原型系统的可行性，该原型系统可以在项目的第二阶段进一步开发为成功的商业产品。如果成功，该项目更广泛的影响/商业潜力将是一个自动化细胞培养实验并消除大部分繁琐的手工劳动的系统。 作为一种低成本、紧凑的诊断工具，它将使在地理上分散的地区进行研究和诊断成为可能，这些地区无法获得集中的细胞培养实验室。 并且，在小学教育实验室中，由于其成本低、操作简单，将向大量高中生介绍细胞培养实验方法和技术。总之，这些影响将增强对细胞生长的科学和技术理解，并为研究，诊断和教育带来新的工具。根据对市场规模和潜在市场份额的现实预测，智能培养皿的潜在商业价值是到生产的第三年每年收入1000万美元。