Collaborative Research: Dissolved Phosphorus Processing by Trichodesmium Consortia: Quantitative Partitioning, Role of Microbial Coordination, and Impact on Nitrogen Fixation

合作研究：Trichodesmium Consortia 的溶解磷处理：定量分配、微生物协调的作用以及对固氮的影响

• 批准号: 1332912
• 负责人: Sonya Dyhrman
• 金额: $ 55.7万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-10-01 至 2018-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1332912&HistoricalAwards=false
• 关键词: Collaborative; Research; Dissolved; Phosphorus; Processing

Colonies of the cyanbacterium Trichodesmium are responsible for a large fraction of N2 fixation in nutrient-poor, open-ocean ecosystems, ultimately fueling primary production in both Trichodesmium and in the broader planktonic community. However, in some parts of the ocean, the scarcity of dissolved phosphorus limits rates of Trichodesmium N2 fixation. Trichodesmium colonies employ an arsenal of strategies to mitigate the effects of phosphorus limitation, and the consortia of epibiotic bacteria in the colonies may play a significant role in phosphorus acquisition. In this study, researchers from Woods Hole Oceanographic Institution and Columbia University will use metagenomic and metatranscriptomic sequencing to investigate how phosphorus metabolism is coordinated in Trichodesmium consortia, and to discern the role of quorum sensing in phosphorus acquisition and partitioning. Results from this study are expected to expand understanding of Trichodesmium from a monospecific colony whose primary function is fixing CO2 and N2 toward a unique planktonic consortium with a diverse, complex, and highly coordinated overall metabolism that exerts profound control over the cycling of inorganic and organic nutrients in the oligotrophic upper ocean.Broader Impacts: This project will provide research experience for an under-represented ethnic graduate student and take advantage of established K-12 standards-based outreach programs to increase ocean literacy in children and amongst the public.

蓝杆菌束毛藻菌落负责营养贫乏的公海生态系统中的大部分N2固定，最终促进束毛藻和更广泛的浮游生物群落的初级生产。然而，在海洋的某些地方，溶解磷的稀缺限制了束毛藻固氮的速率。束毛藻菌落采用一系列策略来减轻磷限制的影响，菌落中的表生细菌聚生体可能在磷获取中起重要作用。在这项研究中，来自伍兹霍尔海洋研究所和哥伦比亚大学的研究人员将使用宏基因组和元转录组测序来研究束毛藻聚生体中磷代谢是如何协调的，并识别群体感应在磷获取和分配中的作用。这项研究的结果有望扩大对束毛藻属的了解，该属是一个单种性的群体，其主要功能是将CO2和N2固定到一个独特的共生体中，该共生体具有多样、复杂和高度协调的整体代谢，对贫营养上层海洋中无机和有机营养物质的循环发挥深刻的控制作用。该项目将为代表性不足的民族研究生提供研究经验，并利用已建立的K-12标准为基础的推广计划，以提高儿童和公众的海洋素养。