Deciphering subsurface microbial activity on the Iberian Margin

破译伊比利亚边缘的地下微生物活动

• 批准号: 1333104
• 负责人: Jennifer Biddle
• 金额: $ 10.5万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1333104&HistoricalAwards=false
• 关键词: Deciphering; subsurface; microbial; activity; Iberian

This proposal seeks to characterize the microbes recovered from sub ocean floor sediments cored during IODP leg 339 and compare them to the well-characterized microbial suite found in similar sediments off the Peru margin. Metatranscriptomic analysis enables the recognition of the active biome by targeting the whole sample messenger RNA and relating the RNA to known gene function. Thus the metabolic behavior and taxonomy of the entire microbial community can be assessed and the relative proportion of active vs. inactive biota (paleome vs. biome) determined. The samples for metatranscriptomic analysis are selected from portions of the core taken from 1.) sulfate-rich areas in the shallow subsurface; 2.) methane-rich samples from the deeper subsurface, and 3.) samples taken from near the sulfate-methane transition. The hypothesis to be tested is that these chemical variations are directly related to sample biology. This work is needed in order for the objectives of expedition 339 to be fully met. Broader impacts include graduate and early career investigator involvement, community outreach, and an IODP lectureship. Scientific impacts include characterization of part of the world?s largest and most poorly understood ecosystem, the sub-seafloor deep biosphere. The potential for discovering new species and metabolic pathways is significant.

该提案旨在确定在国际大洋钻探项目第339航次期间取芯的海底沉积物中回收的微生物的特征，并将其与在秘鲁边缘附近类似沉积物中发现的特征明确的微生物组进行比较。 元转录组学分析通过靶向整个样品信使RNA并将RNA与已知基因功能相关联来识别活性生物组。因此，可以评估整个微生物群落的代谢行为和分类，并确定活性与非活性生物群（古生物群与生物群）的相对比例。用于元转录组学分析的样品选自取自1.）浅层地下富含硫酸盐的区域; 2.）来自更深地下的富含甲烷的样品，以及3.）从硫酸盐-甲烷转换附近采集的样本。待检验的假设是这些化学变化与样品生物学直接相关。 为了充分实现第339次远征的目标，这项工作是必要的。更广泛的影响包括研究生和早期职业调查员的参与，社区推广，和IODP讲师。科学影响包括世界部分地区的特征？世界上最大的、也是最不为人所知的生态系统--海底下的深海生物圈。发现新物种和代谢途径的潜力是巨大的。