EAGER: Profiling Dynamic Changes in Animal Circadian Clock Protein Networks

EAGER：分析动物昼夜节律时钟蛋白质网络的动态变化

• 批准号: 1342603
• 负责人: Joanna Chiu
• 金额: $ 15万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1342603&HistoricalAwards=false
• 关键词: EAGER; Profiling; Dynamic; Changes; Animal

Intellectual Merit:The objective of this EAGER project is to profile the dynamic interactions between circadian oscillator protein compleesx and other cellular machineries by using mass spectrometry-based proteomics and Drosophila melanogaster as a model. This project is to develop foundational data to probe the dynamic rewiring of the circadian interactome necessary for biological timekeeping. Looking ahead, results from this project will lay the foundation for (i) functional characterization of new clock components, and (ii) comparative protein network analysis of the circadian interactomes in multiple species with distinct clock designs. This comparison coupled with rigorous mathematical approaches will highlight network rewiring and differential interactions that can reflect inherent flexibility and adaptability of the clock network.Broader Impacts:Circadian biology is a fascinating topic that people of all ages and backgrounds can relate to, as circadian clocks regulate physiological processes that are of interest to many, and therefore represents a perfect platform for science education. The PI and her colleagues developed a multi-faceted plan to use insect circadian rhythms to educate and inspire future generations of scientists of all ages and engage the general public. This project will also provide cross-disciplinary training and career development opportunities for graduate students, undergraduates, and high school teachers.

智力价值：这个迫切的项目的目标是通过使用基于质谱学的蛋白质组学和黑腹果蝇作为模型来描述昼夜节律振荡器蛋白Compleesx和其他细胞机制之间的动态相互作用。该项目旨在开发基础数据，以探索生物计时所必需的昼夜节律相互作用组的动态重新布线。展望未来，该项目的结果将为(I)新时钟组件的功能表征和(Ii)具有不同时钟设计的多个物种的昼夜节律相互作用的比较蛋白质网络分析奠定基础。这种比较加上严格的数学方法将突出网络重新布线和差异相互作用，这些可以反映时钟网络固有的灵活性和适应性。广泛的影响：昼夜生物学是一个令人着迷的话题，所有年龄和背景的人都可以与之相关，因为生物钟调节着许多人感兴趣的生理过程，因此代表着科学教育的完美平台。PI和她的同事们制定了一个多方面的计划，利用昆虫的昼夜节律来教育和激励未来几代所有年龄段的科学家，并让普通公众参与进来。该项目还将为研究生、本科生和高中教师提供跨学科培训和职业发展机会。