NSF/FDA SIR: Isolation of cancer stem cells and the determination of the role of microRNA in B cell differentiation by flow cytometric techniques

raveche， elizabeth：该方案使用带有特定荧光标签的标记物对单个细胞进行多参数分析，该标记物在激光激发下发出可以与所有其他信号和背景区分开来的信号。通过这种多色分析，精细的小样本可以被识别和最终分类，并用于分子和功能分析。这项拟议的研究将测试该技术定义独特的恶性前体细胞的能力，这将对未来的科学突破至关重要。该提案最初利用了工程上的进步，使荧光染料具有独特的激发和发射概况，从而能够不同地激发和收集这些排放物。分析单个细胞（或颗粒）而不是整个群体，可以检测在同一细胞上测量的多种特性。检测速度很快（与液体鞘中的细胞通过激光束一样快）。除了分析单个细胞外，某些类型的流式细胞仪还可以根据与被检测参数相关的信号对细胞进行物理分选。还有科学研究。

Abstract#1343519Raveche, ElizabethThis proposal employs multiparameter analysis of individual cells using markers with specific fluorescence tags that upon laser excitation emit a signal that can be distinguished from all other signals and background. Through this multi-color analysis exquisitely small samples can be identified and ultimately sorted and employed in molecular and functional assays. . This proposed research will test the ability of this technology to define unique malignant precursor cells that will be important for future scientific breakthroughs. This proposal initially takes advantage of the engineering advancements that have allowed fluorescent dyes with unique excitation and emission profiles to result in the ability to differentially excite and collect these emissions. The analysis of individual cells (or particles) rather than the whole population allows for detection of multiple properties measured on the same cell. The detection is rapid (as fast as the cell in the fluid sheath passes through the laser beam). In addition to analysis of individual cells, some types of flow cytometers can physically sort cells based on signals associated with the parameters being detected. and scientific research.