The role of endoderm in cardiac morphogenesis

姓名（N）：方琳提案编号：IOS-1354457提案标题：内胚层在心脏形态发生中的作用在脊椎动物心脏发育过程中，一个关键的保守步骤是心肌前体的两个双侧群体向胚胎中线迁移，在那里它们合并形成原始心管-心脏发育的基础。这种迁移是高度调节的，需要迁移的心肌前体细胞与其微环境（包括相邻的内胚层）之间的相互作用。然而，内胚层促进心肌迁移的机制尚不清楚。主要研究者的实验室已经确定了一个信号通路，这是内胚层组织层和心肌前体细胞迁移的关键。在这个项目中，他们将应用他们在斑马鱼遗传学，GPCR信号和体内成像方面的专业知识，并使用现有的工具来解剖内胚层和心肌前体之间的相互作用。这项研究有望使我们更清楚地了解内胚层如何调节心肌迁移，这可能广泛适用于其他物种。这一贡献将是显着的，因为它将建立一个框架，内胚层和心肌前体之间的相互作用，在脊椎动物心脏发育，并提供深入了解的机制，组织相互作用影响器官的发展。从这项研究中获得的知识将有助于我们了解心脏是如何发育的。斑马鱼不仅代表了一种有价值的研究模型，也是各级学生的优秀教学工具。斑马鱼胚胎是透明的，外部发育，使得通过简单的立体显微镜观察发育过程，包括心脏发育成为可能。因此，斑马鱼提供了一个可交付的基于课堂的学习机会。PI热衷于将科学带入课堂，激励新一代，并促进公众对基础生物学研究的认识。她认为，这些努力对社会产生了积极影响。PI将继续在当地K-12学校开展外展教育项目，为本科生提供研究经验，并为研究生和博士后奖学金培训做出贡献。

Name: Fang LinProposal number: IOS-1354457Proposal title: The role of endoderm in cardiac morphogenesisDuring vertebrate heart development, a key conserved step is the migration of two bilateral populations of myocardial precursors towards the embryonic midline, where they merge to form the primitive heart tube- the foundation of heart development. This migration is highly regulated and requires interactions between the migrating myocardial precursors and their microenvironment, which includes the adjacent endoderm. However, the mechanisms enabling endoderm to promote myocardial migration are unclear. The Principal Investigator's lab has identified a signaling pathway that is critical for migration of the endodermal tissue layer and the myocardial precursors. In this project, they will apply their expertise in zebrafish genetics, GPCR signaling, and in vivo imaging, and use established tools to dissect the interactions between the endoderm and myocardial precursors. This study is expected to lead to a clear understanding of how the endoderm regulates myocardial migration, which may be broadly applicable to other species. This contribution will be significant in that it will establish a framework for the interplay between the endoderm and myocardial precursors during vertebrate heart development, and provide insights into the mechanisms whereby tissue interactions influence organ development. The knowledge gained from this study will help us to understand how the heart develops.The zebrafish represents not only a valuable research model, but also an excellent teaching tool for students at all levels. The zebrafish embryo is transparent and develops externally, making it possible to observe developmental processes, including heart development, through a simple stereomicroscope. Thus, zebrafish provides a deliverable classroom-based learning opportunity. The PI is passionate about bringing science to the classroom, inspiring new generations, and promoting an awareness of basic biological research among the public. She believes that these efforts have a positive impact on society. The PI will continue to conduct outreach educational projects at local K-12 schools, to provide undergraduates with research experience, and to contribute to graduate and postdoctoral fellowship training.