Biogenic formation of non-extractable residues from pesticides in soil

土壤中农药不可萃取残留物的生物形成

• 批准号: 214367779
• 负责人: Dr. Karolina Malgorzata Nowak
• 金额: --
• 依托单位: Lehrstuhl Umweltbiologie und -chemodynamik (UBC)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/214367779?language=en
• 关键词: Biogenic; formation; non; extractable; residues

During microbial turnover of organic chemicals in soil, non-extractable residues (NER) are formed frequently. Studies on NER formation usually performed with radioisotope labelled tracer compounds are limited to localisation and quantitative analyses but their chemical composition is left unknown. Recently, we could show for 2,4-dichlorophenoxyacetic acid and ibuprofen that during microbial turnover in soil nearly all NER were derived from microbial biomass, since degrading bacteria use the pollutant carbon for their biomass synthesis. Their cell debris is subsequently stabilised within soil organic matter (SOM) forming biogenic NER (bioNER). It is still unknown whether bioNER are also formed during biodegradation of other, structurally different compound classes of organic contaminants. Therefore, agricultural soil will be incubated with labelled compounds of five classes of commonly used and emerging pesticides: organophosphate, phenylurea, triazinone, benzothiadiazine and aryloxyphenoxypropionic acid. The fate of the label will be monitored in both living and non-living SOM pools and the formation of bioNER will be quantified for each compound over extended periods of time. In addition, soil samples from long-term lysimeter studies with 14C-labelled pesticide residues (e.g. triazine, benzothiazole and phenoxypropionic acid group) will be also analysed for bioNER formation. The results will be summarised to identify the metabolic conditions of microorganisms needed for bioNER formation and to develop an extended concept of risk assessment including bioNER formation in soils.

在土壤中有机化学物质的微生物周转过程中，经常会形成不可提取的残留物（NER）。通常使用放射性同位素标记的示踪化合物进行的 NER 形成研究仅限于定位和定量分析，但其化学成分未知。最近，我们可以证明 2,4-二氯苯氧基乙酸和布洛芬在土壤中的微生物周转过程中几乎所有 NER 都来自微生物生物质，因为降解细菌使用污染物碳来合成其生物质。它们的细胞碎片随后稳定在土壤有机质 (SOM) 中，形成生物 NER (bioNER)。目前尚不清楚 bioNER 是否也在其他结构不同的有机污染物化合物类别的生物降解过程中形成。因此，农业土壤将与五类常用和新兴农药的标记化合物一起培育：有机磷酸酯、苯脲、三嗪酮、苯并噻二嗪和芳氧基苯氧基丙酸。标签的命运将在活体和非活体 SOM 池中进行监测，并且在较长时间内对每种化合物的 bioNER 形成进行量化。此外，还将分析来自长期蒸渗仪研究的含有 14C 标记农药残留（例如三嗪、苯并噻唑和苯氧基丙酸基团）的土壤样品，以了解 bioNER 的形成。对结果进行总结，以确定 bioNER 形成所需的微生物的代谢条件，并开发风险评估的扩展概念，包括土壤中 bioNER 的形成。

项目成果

(Bio)degradation of glyphosate in water-sediment microcosms - A stable isotope co-labeling approach.

• DOI: 10.1016/j.watres.2016.04.041
• 发表时间: 2016-08
• 期刊: Water research
• 影响因子: 12.8
• 作者: Shizong Wang;B. Seiwert;M. Kästner;A. Miltner;A. Schäffer;T. Reemtsma;Qi Yang;K. Nowak