Research Initiation Award: Establishment of Pale Yellow Golden Pothos Plant-based Transgenic System for Studying Nuclear Genes Involved in Chloroplast Biogenesis
研究启动奖:建立浅黄色金绿萝植物转基因系统,研究叶绿体生物发生中涉及的核基因
基本信息
- 批准号:1400946
- 负责人:
- 金额:$ 19.47万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-12-15 至 2018-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Research Initiation Awards (RIAs) provide support for junior faculty at Historically Black Colleges and Universities (HBCUs) who are starting to build a research program, as well as for mid-career faculty who need to re-direct and re-build a research program. It is expected that the award helps to further the faculty member's research capability and effectiveness, improves research and teaching at the researcher's home institution, and involves undergraduate students in research experiences.North Carolina Central University's (NCCU) HBCU-UP RIA is aimed at establishing an experimental system to study nuclear genes encoding chloroplast proteins and to test its reliability for the purpose by co-expressing two well-characterized nuclear-encoded plastid protein genes individually. Pale yellow 'Golden Pothos' plants resulting from impaired function of EaZIP, encoding the Mg-protoporphyrin IX monomethyl ester cyclase in the chlorophyll biosynthesis pathway, is used for overexpression by EaZIP driven by an inducible promoter alcA (alcA:: EaZIP). The overall goal of the RIA is to develop a pale yellow 'Golden Pothos' plant-based inducible transgenic system to study chloroplast biogenesis.During the two year duration, the project supports five undergraduate students in biotechnology. The project has the potential for local, regional and national impact through a collaboration with researchers at the University of Florida and the United States Department of Agriculture (USDA). Institutional resources in support of the PI and students are also provided through the BRITE Institute at NCCU. The project outcome also has the potential to result in training opportunities for additional students, particularly undergraduates from underrepresented minority populations, in molecular biology and biotechnology in gene cloning, for example. In addition, the project increases research capability and capacity at a historically black university in addition to making contributions to a diverse, highly competitive, scientific workforce for the nation.
研究启动奖(RIAs)为历史上黑人学院和大学(HBCU)的初级教师提供支持,他们开始建立研究计划,以及需要重新指导和重新建立研究计划的中期教师。 预计该奖项将有助于进一步提高教师的研究能力和效率,改善研究人员所在机构的研究和教学,北卡罗来纳州中央大学(NCCU)的HBCU-UP RIA旨在建立一个研究编码叶绿体蛋白质的核基因的实验系统,并通过共同研究来测试其可靠性。分别表达两个充分表征的核编码质体蛋白基因。将由EaZIP(在叶绿素生物合成途径中编码Mg-原卟啉IX单甲酯环化酶)功能受损产生的淡黄色“Golden Pothos”植物用于通过由诱导型启动子alcA(alcA::EaZIP)驱动的EaZIP过表达。 RIA的总体目标是开发一个淡黄色的“金菩提树”植物诱导转基因系统来研究叶绿体的生物发生。在两年的时间里,该项目支持了五名生物技术专业的本科生。 通过与佛罗里达大学和美国农业部的研究人员合作,该项目有可能对地方、区域和国家产生影响。 支持PI和学生的机构资源也通过NCCU的BRITE研究所提供。 该项目的成果还有可能为更多的学生,特别是来自代表性不足的少数民族的大学生提供分子生物学和基因克隆生物技术等方面的培训机会。 此外,该项目还提高了一所历史悠久的黑人大学的研究能力和能力,并为国家建立一支多样化、高度竞争的科学劳动力队伍做出了贡献。
项目成果
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