EAPSI: Characterization of dissolved free amino acids in hatchery system water - a salmon hatchery case study

EAPSI：孵化场系统水中溶解的游离氨基酸的表征 - 鲑鱼孵化场案例研究

• 批准号: 1415250
• 负责人: Joseph Lemanski
• 金额: $ 0.51万
• 依托单位: Lemanski Joseph R
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1415250&HistoricalAwards=false
• 关键词: EAPSI; Characterization; dissolved; free; amino

For many decades, salmon in the Pacific Northwest have been sought after for a multitude of reasons. Due to regional declines in adults returning to their natal rivers to spawn every year, the rearing and release of hatchery salmonids has been utilized for many decades. Unfortunately, hatchery raised salmon have shown a significant decrease in home stream fidelity and impose an assortment of ecological issues due to increased interaction with wild populations. One proposed explanation for the lack of home stream fidelity in hatchery salmonids is inadequate learning of chemical cues in natal river water. The purpose of this study is to determine if the chemical nature of natal river water is altered by simple, daily hatchery operations. This research will be conducted in collaboration with Dr. Hiroshi Ueda, an expert in dissolved free amino acids (DFAA's) in salmonid imprinting and homing, at the Hokkaido University in Japan.It is well understood that salmon use olfaction to identify distinct chemical cues of natal river water when navigating to their natal rivers to spawn. However, the distinct set, or "bouquet" of chemicals that salmon use for this phenomenon has yet to be identified. Recently, considerable attention has been directed towards DFAA's as a set of molecules that salmon use to navigate and identify their natal rivers. Research has shown that salmonids possess the ability to distinguish between certain water sources based on their DFAA compositions. High-performance liquid chromatography (HPLC) will be used to analyze the content of DFAA's in river water. By analyzing water collected from certain locations within a hatchery setting, it will be determine if current hatchery operations may significantly alter the chemical nature of their source river water. This NSF EAPSI award is funded in collaboration with the Japan Society for the Promotion of Science.

几十年来，太平洋西北部的鲑鱼因多种原因而受到追捧。由于每年返回纳塔尔河流产卵的成年鲑鱼的区域性下降，孵化场鲑鱼的饲养和释放已经使用了几十年。不幸的是，孵化场饲养的鲑鱼显示出显着减少的家庭流保真度和强加的各种生态问题，由于增加与野生种群的相互作用。一个建议的解释缺乏孵化场鲑鱼的家流保真度是不充分的学习，在纳塔尔的河水中的化学线索。本研究的目的是确定纳塔尔河水的化学性质是否会因简单的日常孵化操作而改变。这项研究将与日本北海道大学的溶解性游离氨基酸（DFAA）专家上田博士合作进行。众所周知，鲑鱼在航行到纳塔尔河流产卵时，会利用嗅觉识别纳塔尔河流水的独特化学线索。然而，鲑鱼用于这种现象的独特的一组或“花束”化学物质尚未确定。最近，相当多的注意力已经转向DFAA的作为一组分子，鲑鱼使用导航和识别其纳塔尔的河流。研究表明，鲑鱼具有根据其DFAA成分区分某些水源的能力。采用高效液相色谱法分析河水中DFAA的含量。通过分析从孵化场设置内的某些位置收集的水，将确定当前孵化场操作是否可能显着改变其源河水的化学性质。这个NSF EAPSI奖是与日本科学促进协会合作资助的。