MRI: Acquisition of a Microscope-based, Spectrofluorometer

MRI：获取基于显微镜的分光荧光计

• 批准号: 1427937
• 负责人: Jayashree Sarathy
• 金额: $ 14.14万
• 依托单位: Benedictine University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1427937&HistoricalAwards=false
• 关键词: MRI; Acquisition; Microscope; based; Spectrofluorometer

Benedictine University faculty and student researchers will use the spectrofluorometer/microscope system to initiate projects that involve the detection of rapid changes in the fluorescence state of molecules in response to their chemical environments. This work allows researchers to investigate contemporary issues in the fields of physiology, molecular biology, and biochemistry by using high-tech instrumentation to acquire real-time measures of cell physiology and signaling without damaging the cells. Undergraduates, including those from underrepresented backgrounds, have the opportunity to participate in research and gain a solid foundation for careers or graduate school. This work also supports an internship program involving both pre-service and K-8 in-service teachers, enabling them to learn first-hand the excitement of investigation and achievement in science that they then bring to the next generation of students. Researchers in the biology and chemistry departments at Benedictine University will use the spectrofluorescence system to advance their scientific research programs in four biochemistry projects: (1) Regulation of paracellular permeability and ion transport in epithelial cells; (2) Anti-estrogenic effects of metals in osteoclasts; (3) Structure/function properties of G-protein signaling; (4) Fluorescence resonance energy transfer (FRET)-monitored phage display system for the targeting of signal proteins. By promoting interdisciplinary work at Benedictine, these projects together improve the competencies of students by providing access to classical and state-of-the-art techniques in cellular biology and chemistry, such as in the capstone biochemistry and molecular biology cell laboratory. Students gain first-hand experiences and opportunities to conduct original science, with extensive planning, data analysis and reporting, that can potentially lead to new discoveries. The projects contribute to the integration of science processes and quantitative reasoning with Benedictine's curriculum.

本笃会大学的教师和学生研究人员将使用荧光分光光度计/显微镜系统启动项目，涉及检测分子荧光状态响应其化学环境的快速变化。这项工作使研究人员能够通过使用高科技仪器来研究生理学，分子生物学和生物化学领域的当代问题，以获得细胞生理学和信号传导的实时测量，而不会损坏细胞。本科生，包括那些来自代表性不足的背景，有机会参与研究，并获得职业或研究生院的坚实基础。这项工作还支持涉及职前和K-8在职教师的实习计划，使他们能够直接了解他们然后带给下一代学生的科学调查和成就的兴奋。本笃会大学生物系和化学系的研究人员将利用光谱荧光系统推进他们在四个生物化学项目中的科学研究计划：（1）上皮细胞的细胞旁渗透性和离子转运的调节;（2）金属在破骨细胞中的抗雌激素作用;（3）G蛋白信号的结构/功能特性;（4）G蛋白信号的结构/功能特性。（4）用于靶向信号蛋白的荧光共振能量转移（FRET）监测的噬菌体展示系统。通过促进本笃会的跨学科工作，这些项目通过提供细胞生物学和化学的经典和最先进的技术来提高学生的能力，例如在顶点生物化学和分子生物学细胞实验室。学生获得第一手的经验和机会进行原创科学，广泛的规划，数据分析和报告，这可能会导致新的发现。 这些项目有助于科学过程和定量推理与本笃会课程的整合。