I-Corps: Commercialization of immobilized enzymes, processes and services

I-Corps：固定化酶、工艺和服务的商业化

• 批准号: 1445740
• 负责人: Richard Gross
• 金额: $ 5万
• 依托单位: Rensselaer Polytechnic Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2015-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1445740&HistoricalAwards=false
• 关键词: Corps; Commercialization; immobilized; enzymes; processes

Expertise is needed to develop immobilized enzyme preparations that are cost-effective, enhance enzyme activity and allow multiple reaction cycles. Surprisingly, large enzyme producers are not organized to provide this capability. Their focus is to efficiently manufacture enzymes that meet demands in high volume markets. Similarly, few chemical manufacturers have the internal capability to develop efficient enzyme catalyzed manufacturing processes. The commercial development of immobilized enzyme biocatalyst systems has the potential to provide transformational new technologies to the chemical industry that lower process energy input and avoid protection-deprotection steps. This team has developed deep insight and practical experience in creating superior performance immobilized enzymes. The proposed technology will develop a deep understanding of how enzyme properties, including activity and stability, are influenced by the surface chemistry and physical parameters of immobilization resins. By identifying optimal combinations of enzymes and immobilization resins, important improvements in enzyme activity and stability will be achieved. The proposed new company will also develop insights into resin-enzyme combinations that improve immobilized enzyme biocatalysts for multiple cycle use. A key approach to gain knowledge in the development of high performance immobilized enzyme systems will be the implementation of a high throughput screening process.

需要专门知识来开发具有成本效益的固定化酶制剂，提高酶活性并允许多个反应循环。令人惊讶的是，大型酶生产商并没有组织起来提供这种能力。他们的重点是有效地生产满足大批量市场需求的酶。同样，很少有化学品制造商具有开发有效的酶催化制造工艺的内部能力。 固定化酶生物催化剂系统的商业开发有可能为化学工业提供变革性的新技术，从而降低工艺能量输入并避免保护-脱保护步骤。该团队在制造上级性能固定化酶方面具有深刻的见解和实践经验。 拟议的技术将开发一个深刻的理解，酶的特性，包括活性和稳定性，是如何受到固定化树脂的表面化学和物理参数的影响。通过确定酶和固定化树脂的最佳组合，将实现酶活性和稳定性的重要改进。拟议中的新公司还将深入研究树脂-酶组合，以改善固定化酶生物催化剂的多循环使用。在高性能固定化酶系统的开发中获得知识的一个关键方法是实施高通量筛选过程。