CAREER: Characterizing the Glycoproteome with Effective MS-based Chemical and Enzymatic Methods

获得化学测量和成像计划颁发的这一职业奖后，佐治亚理工学院的吴荣虎教授和他的团队将为复杂生物样本中糖蛋白的全球表征开发新的分析方法。蛋白质糖基化对真核细胞的生存至关重要，几乎参与了每一种细胞事件。基于多糖的共同特征和独特性质，结合化学反应和/或酶反应以及现代基于质谱学的蛋白质组学技术，将设计新的策略来特异性和全面地分析糖蛋白。所提出的方法将为系统研究酵母细胞中糖基化对蛋白质分泌的调节提供一个独特的机会，这将导致对蛋白质糖基化功能的深入了解。这些新开发的方法将在生物和生化研究领域得到广泛应用，并推动糖科学的进步。拟议的研究可能会对糖蛋白研究领域以及整个生物/生化研究界产生重大影响。拟议的研究和教育活动还将为研究生和本科生，包括来自历史上代表性不足的群体的学生，提供这一高度跨学科研究的培训机会，包括分析化学、化学生物学、仪器和大规模数据分析。此外，通过佐治亚理工学院整合科学、数学和计算的教育中心，PI和他的小组将积极参与与高中化学教师和学生的外联活动。

With this CAREER award from the Chemical Measurement and Imaging Program, Professor Ronghu Wu and his group at Georgia Institute of Technology will develop novel analytical methods for the global characterization of glycoproteins in complex biological samples. Protein glycosylation is essential to the survival of eukaryotic cells and is involved in nearly every cellular event. Based on the common features and unique properties of glycans, new strategies integrating chemical and/or enzymatic reactions and modern mass spectrometry-based proteomics techniques will be designed to specifically and comprehensively analyze glycoproteins. The proposed methods will provide a unique opportunity to systemically study the regulation of protein secretion by glycosylation in yeast cells, which will result in an in-depth understanding of the functions of protein glycosylation. The newly developed methods will find extensive applications in biological and biochemical research communities and promote the progress of glycoscience. The proposed research could have a major impact in the field of glycoprotein studies and in the biology/biochemistry research community in general. The proposed research and educational activities will also provide graduate and undergraduate students, including those from historically underrepresented groups, training opportunities in this highly interdisciplinary research including analytical chemistry, chemical biology, instrumentation and large-scale data analysis. In addition, through Georgia Tech's Center for Education Integrating Science, Mathematics and Computing, the PI and his group will actively participate in outreach activities with high school chemistry teachers and students.