CAREER: Characterizing the Glycoproteome with Effective MS-based Chemical and Enzymatic Methods

获得化学测量与成像项目颁发的CAREER奖，佐治亚理工学院的吴荣虎教授和他的团队将开发新的分析方法，用于复杂生物样品中糖蛋白的全局表征。蛋白质糖基化对真核细胞的生存至关重要，几乎涉及到每一个细胞事件。基于糖蛋白的共同特征和独特性质，结合化学和/或酶反应和基于现代质谱的蛋白质组学技术，将设计新的策略来专门和全面地分析糖蛋白。所提出的方法将为系统研究酵母细胞中糖基化对蛋白质分泌的调节提供一个独特的机会，这将导致对蛋白质糖基化功能的深入了解。这些新方法将在生物和生化研究领域得到广泛应用，并促进糖科学的发展。拟议的研究可能会对糖蛋白研究领域和生物学/生物化学研究界产生重大影响。拟议的研究和教育活动还将为研究生和本科生，包括那些历史上代表性不足的群体，提供这一高度跨学科研究的培训机会，包括分析化学，化学生物学，仪器仪表和大规模数据分析。此外，通过佐治亚理工学院整合科学、数学和计算机的教育中心，PI和他的团队将积极参与与高中化学教师和学生的推广活动。

With this CAREER award from the Chemical Measurement and Imaging Program, Professor Ronghu Wu and his group at Georgia Institute of Technology will develop novel analytical methods for the global characterization of glycoproteins in complex biological samples. Protein glycosylation is essential to the survival of eukaryotic cells and is involved in nearly every cellular event. Based on the common features and unique properties of glycans, new strategies integrating chemical and/or enzymatic reactions and modern mass spectrometry-based proteomics techniques will be designed to specifically and comprehensively analyze glycoproteins. The proposed methods will provide a unique opportunity to systemically study the regulation of protein secretion by glycosylation in yeast cells, which will result in an in-depth understanding of the functions of protein glycosylation. The newly developed methods will find extensive applications in biological and biochemical research communities and promote the progress of glycoscience. The proposed research could have a major impact in the field of glycoprotein studies and in the biology/biochemistry research community in general. The proposed research and educational activities will also provide graduate and undergraduate students, including those from historically underrepresented groups, training opportunities in this highly interdisciplinary research including analytical chemistry, chemical biology, instrumentation and large-scale data analysis. In addition, through Georgia Tech's Center for Education Integrating Science, Mathematics and Computing, the PI and his group will actively participate in outreach activities with high school chemistry teachers and students.