UNS:Photomodulation of Forster Cycle in a Fluorescent Protein

PI：Kalkan，Kaan Proposal No：1512157该项目寻求开发新型光学探头，这种探头在切换时速度更快、效率更高，同时减少光漂白的影响。他们将研究金属纳米颗粒和荧光蛋白之间能量转移的光物理，以开发用于超分辨率显微镜应用的稳定的可光激活探测器。假设的光开关是基于使用表面增强光泵浦的FP的Forster循环中的中间态的光创造。这种对Forster循环的光调制将通过单分子表面增强拉曼散射和显微光学光谱来验证和研究。这项工作将开发一种以绿色荧光蛋白(GFP)-银NP偶联物为原型的光开关。光调制是基于激发态质子转移使光吸收从395 nm红移到490 nm，从而使GFP的中间形式(I-形式)的光布居。吸收中的这种光谱漂移反过来有望通过共振能量转移到FP来调制等离子体NP的Mie散射。

PI: Kalkan, KaanProposal No.: 1512157This project seeks to develop new types of optical probes that are faster and more efficient at switching, while reducing effects of photobleaching. They will study the photophysics of energy transfer between metal nanoparticles and fluorescent proteins to develop stable photoactivatable probes for super-resolution microscopy applications.The hypothesized photoswitching is based on photocreation of the intermediate state in the Forster cycle of the FP using surface-enhanced optical pumping. This photomodulation of the Forster cycle will be verified and studied by single molecule surface-enhanced Raman scattering and micro-optical spectroscopy. This effort will develop a photoswitch using Green Fluorescent Protein (GFP) - silver NP conjugates as its prototype. The photomodulation is based upon photopopulation of the intermediate form (I-form) of GFP by excited state proton transfer that red-shifts the optical absorption from 395 to 490 nm. This spectral shift in absorption, in turn, is expected to modulate the Mie scattering of the plasmonic NP by resonant energy transfer to the FP.