Identification and characterization of early interleukin (IL)-5/IL-13-producing cells and their inducing factors in experimental pulmonary cryptococcosis

辅助性T (Th) 2细胞反应与对寄生虫的保护性免疫有关。在免疫病理学方面，它们在过敏和支气管哮喘等疾病中很重要。在真菌病原体新型隐球菌感染的肺模型中，还发现Th2细胞诱导的免疫病理表现为典型的杯状细胞增生、嗜酸性粒细胞增多、支气管收缩和巨噬细胞交替活化。早期产生白细胞介素(IL)-5/-13的细胞先于免疫病理Th2细胞的激活。该项目的目标之一是在实验性肺隐球菌病中鉴定这些Th2细胞因子产生细胞。如果最近发现的th2相关细胞因子（IL-25, IL-33）也在隐球菌病中发现，调节早期IL-5/ il -13产生细胞，这也是一个有趣的问题。本项目获得的结果将拓宽我们对新生梭菌与先天免疫细胞相互作用的认识，以及它们释放的介质对诱导适应性Th2反应的影响。

T helper (Th) 2 cell-responses are associated with protective immunity against parasites. In terms of immunopathology they can be important in diseases like allergy and Asthma bronchiale. In a pulmonary model of infection with the fungal pathogen Cryptococcus neoformans Th2 cell-induced immunopathology with typical goblet cell hyperplasia, eosinophilia, bronchconstriction and alternatively activated macrophages was found, too. Early interleukin (IL)-5/-13-producing cells precede the activation of immunopathological Th2 cells. One of the goals of the proposed project is to identify these Th2 cytokine-producing cells in experimental pulmonary cryptococcosis. It is also of interest if recently discovered Th2-associated cytokines (IL-25, IL-33), which are also found in cryptococcosis, regulate the early IL-5/IL-13-producing cells. The obtained results from this project will broaden our knowledge about the interaction of C. neoformans with innate immune cells and the impact of their released mediators on induction of adaptive Th2 responses.