EAPSI: Investigating the regulatory Mechanisms of Plant Thimet Oligopeptidases

EAPSI：研究植物硫美特寡肽酶的调控机制

• 批准号: 1514947
• 负责人: Timothy Westlake
• 金额: $ 0.51万
• 依托单位: Westlake Timothy J
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1514947&HistoricalAwards=false
• 关键词: EAPSI; Investigating; regulatory; Mechanisms; Plant

Plant diseases result in multi-billion dollar crop loss worldwide. Salicylic acid (SA) is a vital plant hormone that plays a central role in immune signaling and activation of defense responses. The hormone elicits a cascade of effects that regulates gene expression and protein activity. The project explores the means in which the activity and protein-interactions of two salicylic acid binding proteins, thimet oligopeptidase 1 and 2 (TOP1 & TOP2) are regulated. The research project will be conducted at RIKEN institute in Yohokama, Japan. The project will be a collaborative effort with Dr. Ken Shirasu, who is at the forefront of research pertaining to plant immunity and salicylic acid. Dr. Shirasu belongs to the renowned RIKEN research institute which houses a state-of-the-art research center with all the equipment and facilities needed to conduct the research project.The project will investigate the enzymatic activity and multimer formation of TOP1 and TOP2. Evidence suggests that TOP activity is governed by redox-sensitive bonds; however, the extent and dynamics of the redox-regulated and hormone-regulated peptide processing of TOP1 and TOP2 have yet to be determined. The objective of the project is to investigate the key components of the proteins that dictate the perceptivity of the peptidases towards SA and the cellular redox state. In order to do this, the activities and oligomerization of TOPs and various TOP deletion/substitution mutants will be assessed under different SA concentrations and reducing/oxidizing environments. The findings will further our understanding of a potential hub of hormone-controlled and redox-regulated peptidase processing in plants. This NSF EAPSI award is funded in collaboration with the Japan Society for the Promotion of Science (JSPS).

植物病害导致全球数十亿美元的农作物损失。水杨酸 (SA) 是一种重要的植物激素，在免疫信号传导和防御反应激活中发挥着核心作用。这种激素会引发一系列调节基因表达和蛋白质活性的效应。该项目探索了两种水杨酸结合蛋白、蒂美特寡肽酶 1 和 2（TOP1 和 TOP2）的活性和蛋白质相互作用的调节方法。该研究项目将在日本横滨的理化学研究所进行。该项目将由 Ken Shirasu 博士合作完成，他处于植物免疫和水杨酸研究的前沿。 Shirasu 博士属于著名的 RIKEN 研究所，该研究所拥有最先进的研究中心，拥有开展研究项目所需的所有设备和设施。该项目将研究 TOP1 和 TOP2 的酶活性和多聚体形成。有证据表明 TOP 活性受氧化还原敏感键控制；然而，TOP1 和 TOP2 的氧化还原调节和激素调节肽加工的程度和动态尚未确定。该项目的目标是研究决定肽酶对 SA 和细胞氧化还原状态的感知能力的蛋白质的关键成分。为此，将在不同的 SA 浓度和还原/氧化环境下评估 TOP 和各种 TOP 缺失/取代突变体的活性和寡聚化。这些发现将进一步加深我们对植物中激素控制和氧化还原调节肽酶加工的潜在中心的了解。该 NSF EAPSI 奖项是与日本科学振兴会 (JSPS) 合作资助的。