SBIR Phase I: The Advancement of a Novel High Efficiency Single Cell Protein for Aquafeeds

SBIR 第一阶段：用于水产饲料的新型高效单细胞蛋白的进展

• 批准号: 1520189
• 负责人: Larry Feinberg
• 金额: $ 15万
• 依托单位: KnipBio Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1520189&HistoricalAwards=false
• 关键词: SBIR; Phase; Advancement; Novel; Efficiency

The broader impact/commercial potential of this Small Business Innovative Research (SBIR) project is to develop an alternative to fishmeal for the fast growing aquaculture industry. Fishmeal is the main component of aquafeed, and there is an urgent need to find alternatives. Aquaculture, the fastest growing animal protein sector, is poised to double by 2030, and represents one of the most effective ways to feed the world's growing population. Two out of every three fish consumed will be raised on a farm. Captured fisheries and aquaculture supplied approximately 154 M tons of fish in 2011 with a total value of $217B. Farm raised fish outpaced beef production in 2013 for the first time ever. The overexploitation of 75% of our natural fisheries has created shrinking supplies of the critical feed ingredient, fishmeal. The continued growth of the industry is vitally tied to generating innovative protein alternatives. In addition, carotenoids like astaxanthin, lycopene, and beta-carotene, provide pigment to color important farm-raised species, but also have an essential nutritional role for immunity as a powerful anti-oxidant. While feed represents the single largest operating cost to the farmer, carotenoids can be the most expensive ingredient within aquafeeds.This SBIR Phase I project proposes to develop engineered, methanol-utilizing bacteria that convert natural gas into a novel carotenoid-laden single cell protein capable of dramatically lowering the cost of carotinoids, and serving as a suitable replacement for fishmeal. The goal is to commercialize an economically viable source of carotenoids, amino acids, and alternative protein. The project uses advanced genetic tools that enable rapid iteration for strain development to meet the goals of increasing efficiency by 50%. The economical co-production of protein and carotenoids leads to a healthier, advantaged feed, while combining high-value functional ingredients with high-efficiency bioconversion.

小企业创新研究（SBIR）项目的更广泛影响/商业潜力是为快速增长的水产养殖业开发鱼粉替代品。 鱼粉是水产饲料的主要成分，迫切需要找到替代品。 水产品是增长最快的动物蛋白行业，到2030年将翻一番，是养活世界不断增长的人口的最有效方式之一。 每三条鱼中就有两条是在农场饲养的。2011年，捕捞渔业和水产养殖提供了约1.54亿吨鱼，总价值为2.17亿美元。2013年，养殖鱼类产量首次超过牛肉产量。我们75%的天然渔业资源被过度开发，导致关键饲料成分鱼粉的供应减少。该行业的持续增长与产生创新的蛋白质替代品密切相关。此外，类胡萝卜素如虾青素、番茄红素和β-胡萝卜素，为重要的农场养殖物种提供色素，但作为一种强大的抗氧化剂，对免疫力也具有重要的营养作用。虽然饲料是养殖户最大的经营成本，但类胡萝卜素可能是水产饲料中最昂贵的成分。SBIR第一阶段项目提出开发利用甲醇的工程菌，将天然气转化为一种新型的含类胡萝卜素的单细胞蛋白，能够显著降低类胡萝卜素的成本，并作为鱼粉的合适替代品。其目标是将经济上可行的类胡萝卜素、氨基酸和替代蛋白质来源商业化。该项目使用先进的遗传工具，使菌株开发能够快速迭代，以实现将效率提高50%的目标。经济的蛋白质和类胡萝卜素的联合生产导致更健康，更健康的饲料，同时结合高价值的功能性成分与高效的生物转化。