STTR Phase I: Development of Modular Selectivity High-Pressure Liquid Chromatography Bonded Phases

这个小型企业技术转让(STTR)第一阶段项目旨在通过生产用于高压和超高压液相色谱(高效液相/超高压)的新型和改进的相，来开发用于分析化学的改进仪器。这项工作将开发一条基于已知聚合物化学的基础上建立在底层相上的不同表面化学的合成路线，该化学是稳定的和易于进一步功能化的。由此产生的化学反应将以柱和固相萃取(SPE)装置的形式提供一系列新的高效液相产品，表现出与商业产品显著不同的分离选择性。这些新相还将具有对亲水性分析物的高选择性，以及手性和几何异构体的选择性。这项技术将提供更大的动力来实现困难的分离，并增加了定制阶段的可能性。所设想的新阶段预计将立即在代谢组学和药物应用领域产生应用。这项拟议的研究将帮助其他利用高效液相/超高效液相分离的市场部门，包括环境、临床和法医分析。据估计，2015年河北石化耗材行业的规模将达到37亿美元。该项目的智力优势包括将经过验证的聚合物技术应用于另一个领域。粘结在二氧化硅表面的聚合物技术提供了一种模板，在模板上，各种有机部分可以“咬合”到聚合物类型的网络中，以产生最终产品。将这项技术应用于色谱相提供了一个巨大的机会，通过提供真正新的和独特的高效液相色谱相，包括反相、亲水相互作用液相色谱(HILIC)、手性相和大分子相。应用领域很多，如果需要，可以很容易地从分析工作扩展到准备工作。化学成分的简单加上原材料的低成本将以相对较低的价格提供种类繁多的产品。在这项第一阶段的工作中，将开发和提炼三种原型相类型(烷基、芳基和手性)。将使用不同的模型药物分析物来证明选择性的独特性。代谢组学的应用也将被评估。这项工作将证明相稳定性和柱性能相当于或优于类似的商业高效液相产品。预计高质量的高效液相色谱柱技术将提供具有显著变化的选择性的强大的可重现性产品。

This Small Business Technology Transfer (STTR) Phase I project is aimed at the development of improved instrumentation for analytical chemistry, via the production of novel and improved phases for high-pressure and ultra-high-pressure liquid chromatography (HPLC/UHPLC). This effort will develop a synthetic route to diverse surface chemistries built on an underlying phase based on known polymer chemistry which is stable and easy to further functionalize. The resultant chemistries will provide a new series of HPLC products, in the form of columns and solid phase extraction (SPE) devices, exhibiting separation selectivities which are significantly different from commercially available products. These new phases will also feature high selectivity for hydrophilic analytes, and chiral and geometric isomer selectivity. This technology will provide greater power to achieve difficult separations with the added possibility of customizable phases. The envisioned new phases are expected to have immediate applicability in the areas of metabolomics and pharmaceutical applications. The proposed research will aid other market sectors utilizing HPLC/UHPLC separations including environmental, clinical and forensic analysis. The HPLC consumables industry is estimated to reach $3.7 billion in 2015. The intellectual merit of this project involves the use of proven polymer technology applied to an alternate field. The polymer technology bonded to a silica surface offers a template upon which a large variety of organic moieties may be "snapped" into the polymer-type network to yield the final products. Applying this technology to chromatographic phases presents a tremendous opportunity to enhance the field by providing truly new and unique HPLC phases including reversed phase, hydrophilic interaction liquid chromatography (HILIC), chiral and macromolecule phases. Application areas are many and can be easily scalable from analytical to preparative work if desired. The simplicity of the chemistry coupled with the low cost of raw materials will provide a diverse product set at relatively low price. In this Phase I work, three prototype phase types (alkyl, aryl, and chiral) will be developed and refined. Uniqueness of selectivity will be demonstrated using diverse sets of model pharmaceutical analytes. Metabolomics applications will also be evaluated. This work will demonstrate phase robustness and column performance to be equivalent to or better than similar commercially available HPLC products. The anticipation is that quality HPLC column technology will result offering robust reproducible products with dramatically altered selectivity.