STTR Phase I: Development of Modular Selectivity High-Pressure Liquid Chromatography Bonded Phases

这项小型企业技术转移（STTR）第一期项目旨在通过生产高压和超高压液相色谱（HPLC/UHPLC）的新型和改进相，开发改进的分析化学仪器。这项工作将开发一种基于已知聚合物化学的底层相的多种表面化学合成途径，这种相稳定且易于进一步功能化。由此产生的化学物质将提供一系列新的高效液相色谱产品，以柱和固相萃取（SPE）设备的形式，表现出与市售产品显著不同的分离选择性。这些新相还具有对亲水分析物的高选择性，以及手性和几何异构体的选择性。该技术将提供更大的功率来实现困难的分离，并增加了可定制相位的可能性。预期的新阶段将在代谢组学和制药应用领域具有直接适用性。拟议的研究将有助于其他市场部门利用HPLC/UHPLC分离，包括环境，临床和法医分析。HPLC耗材行业预计在2015年将达到37亿美元。该项目的智力优势在于将成熟的聚合物技术应用于替代领域。结合到二氧化硅表面的聚合物技术提供了一个模板，在该模板上，各种各样的有机部分可以被“折断”到聚合物型网络中，以产生最终产品。将该技术应用于色谱相提供了一个巨大的机会，通过提供真正新的和独特的HPLC相，包括反相，亲水相互作用液相色谱（HILIC），手性和大分子相来增强该领域。应用领域很多，如果需要，可以很容易地从分析工作扩展到准备工作。简单的化学反应加上低成本的原材料将以相对较低的价格提供多样化的产品。在第一阶段的工作中，将开发和完善三种原型相类型（烷基、芳基和手性）。选择性的独特性将被证明使用不同的模型药物分析。代谢组学的应用也将被评估。这项工作将证明相鲁棒性和色谱性能相当于或优于类似的市售HPLC产品。预计高质量的高效液相色谱柱技术将导致提供具有显著改变选择性的稳健可重复性产品。

This Small Business Technology Transfer (STTR) Phase I project is aimed at the development of improved instrumentation for analytical chemistry, via the production of novel and improved phases for high-pressure and ultra-high-pressure liquid chromatography (HPLC/UHPLC). This effort will develop a synthetic route to diverse surface chemistries built on an underlying phase based on known polymer chemistry which is stable and easy to further functionalize. The resultant chemistries will provide a new series of HPLC products, in the form of columns and solid phase extraction (SPE) devices, exhibiting separation selectivities which are significantly different from commercially available products. These new phases will also feature high selectivity for hydrophilic analytes, and chiral and geometric isomer selectivity. This technology will provide greater power to achieve difficult separations with the added possibility of customizable phases. The envisioned new phases are expected to have immediate applicability in the areas of metabolomics and pharmaceutical applications. The proposed research will aid other market sectors utilizing HPLC/UHPLC separations including environmental, clinical and forensic analysis. The HPLC consumables industry is estimated to reach $3.7 billion in 2015. The intellectual merit of this project involves the use of proven polymer technology applied to an alternate field. The polymer technology bonded to a silica surface offers a template upon which a large variety of organic moieties may be "snapped" into the polymer-type network to yield the final products. Applying this technology to chromatographic phases presents a tremendous opportunity to enhance the field by providing truly new and unique HPLC phases including reversed phase, hydrophilic interaction liquid chromatography (HILIC), chiral and macromolecule phases. Application areas are many and can be easily scalable from analytical to preparative work if desired. The simplicity of the chemistry coupled with the low cost of raw materials will provide a diverse product set at relatively low price. In this Phase I work, three prototype phase types (alkyl, aryl, and chiral) will be developed and refined. Uniqueness of selectivity will be demonstrated using diverse sets of model pharmaceutical analytes. Metabolomics applications will also be evaluated. This work will demonstrate phase robustness and column performance to be equivalent to or better than similar commercially available HPLC products. The anticipation is that quality HPLC column technology will result offering robust reproducible products with dramatically altered selectivity.