Cytoskeletal interactions in axonal transport

轴突运输中的细胞骨架相互作用

• 批准号: 22177883
• 负责人: Professor Dr. Eckhard Mandelkow
• 金额: --
• 依托单位: Klinik für Neurodegenerative Erkrankungen und Gerontopsychiatrie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/22177883?language=en
• 关键词: Cytoskeletal; interactions; axonal; transport

The aim of this project is to analyze the interactions between neuronal motor proteins, microtubules, and microtubule-associated proteins, it will encompass structural studies of the proteins, observations of microtubule-dependent traffic in neurons, analysis of traffic regulation by phosphorylation and of the interactions between the neuronal microtubule and actin cytoskeleton. By combining our expertise in the cell biology, culture and adenoviral transfection of neuronal cells with the expertise of the groups in Hannover-Braunschweig in the areas of advanced imaging techniques and the components of the actomyosin-based cytoskeleton, we will be able to dissect the crosstalk between the filament systems and gain new insights in the cell biology of neuronal cells in much greater detail than previously possible, A special focus will be the question of how tau, an axonal microtubule-associated protein that plays a role in Alzheimer neurodegeneration, can impose a general inhibition of anterograde traffic of vesicles and organelles without being subject to the same inhibition. This requires an analysis of the different modes and motors of cytoskeletal movements in neuronal cell processes.

该项目的目的是分析神经元运动蛋白、微管和微管相关蛋白之间的相互作用，它将包括蛋白质的结构研究，神经元中微管依赖性交通的观察，磷酸化交通调节的分析以及神经元微管和肌动蛋白细胞骨架之间的相互作用。通过将我们在细胞生物学、神经元细胞培养和腺病毒转染方面的专业知识与汉诺威-布伦瑞克研究组在先进成像技术和基于肌动球蛋白的细胞骨架组分方面的专业知识相结合，我们将能够剖析细丝系统之间的串扰，并获得比以前更详细的神经元细胞细胞生物学的新见解，一个特别的焦点将是如何tau，轴突微管相关蛋白，在阿尔茨海默氏症神经退行性变中发挥作用的问题，可以施加一个一般的抑制囊泡和细胞器的顺行交通没有受到相同的抑制。这需要分析神经元细胞过程中细胞骨架运动的不同模式和马达。

项目成果

Microtubule Affinity Regulating Kinase Activity in Living Neurons Was Examined by a Genetically Encoded Fluorescence Resonance Energy Transfer/Fluorescence Lifetime Imaging-based Biosensor

通过基因编码的荧光共振能量转移/荧光寿命成像生物传感器检查调节活神经元激酶活性的微管亲和力

• DOI: 10.1074/jbc.m111.257865
• 发表时间: 2011
• 期刊: The Journal of Biological Chemistry
• 作者: von Kries;Zempel;Mandelkow;Mandelkow
• 通讯作者: Mandelkow

Novel diffusion barrier for axonal retention of Tau in neurons and its failure in neurodegeneration

• DOI: 10.1038/emboj.2011.376
• 发表时间: 2011-11-30
• 期刊: EMBO JOURNAL
• 影响因子: 11.4
• 作者: Li, Xiaoyu;Kumar, Yatender;Mandelkow, Eckhard
• 通讯作者: Mandelkow, Eckhard