EAPSI: The Effect of Elevated Nitrogen on Lignin Degrading Microbial Communities in Mt. Changbai Soil

EAPSI：增氮对长白山土壤木质素降解微生物群落的影响

• 批准号: 1614272
• 负责人: Hannah Woo
• 金额: $ 0.04万
• 依托单位: Woo Hannah
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1614272&HistoricalAwards=false
• 关键词: EAPSI; Effect; Elevated; Nitrogen; Lignin

This project will investigate the effect of China's pollution on plant litter decomposition. Plant litter consists of dead plant foliage, wood, and roots. Its turnover is an essential process in maintaining organic carbon within soils and is facilitated largely by microorganisms. The unprecedented levels of nitrogen in China due to industrial pollution have drastically increased soil nitrogen concentration. It is unclear how the elevated nitrogen will effect plant litter decomposition and the native microbial communities. Creating laboratory incubations of Mt Changbai field site soil amended with nitrogen, researchers can monitor changes in the composition of plant litter and the microbes involved in plant litter turnover. This research will be conducted in collaboration Dr. Xudong Zhang, a noted expert in soil geochemistry, at the Chinese Academy of Science Institute of Applied Ecology (CAS IAE). Researchers aim to elucidate the long-term impact of elevated nitrogen on forest soil ecosystems because they are important reservoirs of carbon.The project will investigate the effect of elevated nitrogen deposition on the plant litter-degrading microbial community in Mt. Changbai forest soil. This experiment will directly test for the link between stable soil organic carbon lignin and nitrogen deposition. Fresh soil from a field site in the Mt. Changbai will be sampled and amended with lignin and varying concentrations of ammonium nitrate. During incubation, all soil microcosms will be routinely sampled for degraded lignin analysis using alkaline copper oxide. The microcosms will also be sampled weekly for microbial community structure and function analysis via next generation sequencing. Using bioinformatics, samples will be compared to determine differentially abundant species in lignin microcosms at the different levels of nitrogen addition. A better understanding of nitrogen and carbon cycling will lead to better management practices of soil carbon.This award under the East Asia and Pacific Summer Institutes program supports summer research by a U.S. graduate student and is jointly funded by NSF and the Chinese Ministry of Science and Technology.

该项目将调查中国污染对植物凋落物分解的影响。 植物凋落物包括枯死的植物叶子、木头和根。 它的周转是维持土壤中有机碳的一个重要过程，微生物在很大程度上促进了这一过程。 由于工业污染，中国前所未有的氮水平急剧增加了土壤氮浓度。 目前还不清楚氮的增加将如何影响植物凋落物的分解和原生微生物群落。 通过在长白山野外土壤中添加氮，建立实验室培养物，研究人员可以监测植物凋落物成分和参与植物凋落物周转的微生物的变化。这项研究将与中国科学院应用生态研究所（CAS IAE）的著名土壤地球化学专家张旭东博士合作进行。研究人员的目标是阐明氮含量升高对森林土壤生态系统的长期影响，因为它们是重要的碳库。该项目将调查氮含量升高对山中植物凋落物降解微生物群落的影响。长白森林土壤。本实验将直接测试稳定的土壤有机碳木质素和氮沉降之间的联系。 新鲜的土壤从现场在山。将对长白山进行取样，并用木质素和不同浓度的硝酸铵进行改良。在培养过程中，将对所有土壤微观世界进行常规取样，使用碱性氧化铜进行降解木质素分析。每周还将对微宇宙进行采样，通过下一代测序进行微生物群落结构和功能分析。 使用生物信息学，样品将进行比较，以确定在不同水平的氮添加在木质素微宇宙中的差异丰富的物种。更好地理解氮和碳循环将导致更好的土壤碳管理实践。东亚和太平洋夏季研究所计划下的这个奖项支持美国研究生的夏季研究，由NSF和中国科技部共同资助。