EAPSI: Impacts of Levels of a Pesticide Mixture on Hormones and Behavior in the World?s only Self-Fertilizing Fish

EAPSI：农药混合物的水平对世界上唯一的自体受精鱼的激素和行为的影响

• 批准号: 1614364
• 负责人: Lauren Gillespie
• 金额: $ 0.54万
• 依托单位: Gillespie Lauren M
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-15 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1614364&HistoricalAwards=false
• 关键词: EAPSI; Impacts; Levels; Pesticide; Mixture

The only self-fertilizing vertebrate, the mangrove killifish, serves as an indicator of environmental pollution in disappearing wild mangrove habitat. The research will explore varying lengths of exposure to biologically and environmentally relevant levels of mixtures of insecticides, fungicides, and herbicides on aggressive behavior, contest dynamics, testosterone (T), and cortisol (F) in the mangrove killifish (K. marmoratus) utilizing a novel experimental approach analyzing flexibility in behavioral and hormonal response phenotypes. The research will be conducted in collaboration with Dr. Yuying Hsu of National Normal Taiwan University in Taipei, Taiwan. The Hsu lab houses captive populations of the mangrove killfish, offering a unique opportunity for international collaboration. The research augments dissertation research examining behavior and hormones in golf course living birds. Using the killifish as a model, the research in Taiwan will examine how pesticide mixtures commonly in use at this, and other, golf courses may influence behavior and hormones expanding upon my dissertation work. This research may inform on impacts (if any) of levels of contaminants currently thought to have minimal physiological impacts. Studying a unique species while fostering international collaboration with a world leader in scientific research will be valuable to my personal and professional development. Genetic control with predictable relationships between aggressive behavior, testosterone, and cortisol regarding responses to conspecific conflict are well studied in the mangrove killifish. This provides valuable and difficult to achieve control of genetic variation, often thought responsible for individual variation in hormone-behavior relationships. The experimental design is unique, as fish will be exposed to environmentally relevant levels of a pesticide mixture. Exposure will occur for short (7 days) and long intervals (14 days); while traditional methodology typically uses shorter exposure periods this is unrealistic compared to what is experienced naturally. Measuring aggression, testosterone, and cortisol after exposure also deviates from traditional endpoints measured in laboratory contaminant studies. By utilizing novel animal models and toxicological endpoints, in concert with biological methodology, and accounting for synergistic impacts of multiple contaminants, a realistic perspective concerning sensitive impacts of contaminants on physiology and behavior is possible. Interdisciplinary approaches combining behavioral endocrinology and toxicology are necessary for understanding how contaminants may impact behavioral and hormonal profiles, and ultimately, the health of human and wildlife populations.This award under the East Asia and Pacific Summer Institutes program supports summer research by a U.S. graduate student and is jointly funded by NSF and the Ministry of Science and Technology of Taiwan.

红树林鳉鱼是唯一一种自我繁殖的脊椎动物，它是正在消失的野生红树林栖息地环境污染的一个指标。本研究将探讨暴露于生物和环境相关水平的杀虫剂、杀真菌剂和除草剂混合物的不同时间对红树林鳉鱼（K. marmoratus），利用新的实验方法分析行为和激素反应表型的灵活性。这项研究将与台湾台北国立师范大学的Yuying Hsu博士合作进行。徐实验室圈养了大量的红树林红鱼，为国际合作提供了一个独特的机会。 这项研究增加了论文研究，研究高尔夫球场活鸟的行为和激素。使用作为一个模型，在台湾的研究将探讨如何农药混合物常用在这个，和其他，高尔夫球场可能会影响行为和激素扩展后，我的论文工作。这项研究可能会对目前认为具有最小生理影响的污染物水平的影响（如果有的话）提供信息。研究一个独特的物种，同时促进与世界科学研究领导者的国际合作，对我的个人和职业发展都很有价值。遗传控制与可预测的关系之间的攻击行为，睾酮，皮质醇对同种冲突的反应进行了很好的研究，在红树林？这提供了有价值的和难以实现的遗传变异的控制，通常被认为是负责个体变异的行为关系。实验设计是独特的，因为鱼类将暴露于环境相关水平的农药混合物。接触时间有短（7天）和长（14天）两种;传统方法通常使用较短的接触时间，但与自然接触相比，这是不现实的。暴露后测量攻击性、睾酮和皮质醇也偏离了实验室污染物研究中测量的传统终点。通过利用新的动物模型和毒理学终点，与生物学方法相结合，并考虑多种污染物的协同影响，可以从现实的角度看待污染物对生理和行为的敏感影响。结合行为内分泌学和毒理学的跨学科方法对于了解污染物如何影响行为和激素特征，最终影响人类和野生动物种群的健康是必要的。该奖项是东亚和太平洋夏季研究所计划下的一个美国研究生的夏季研究，由NSF和台湾科技部共同资助。