MRI: Acquisition of a Nucleic Acid Analyzer for Research and Teaching

MRI：购买核酸分析仪用于研究和教学

• 批准号: 1624654
• 负责人: Michael Van Dyke
• 金额: $ 6.51万
• 依托单位: Kennesaw State University Research and Service Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2019-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1624654&HistoricalAwards=false
• 关键词: MRI; Acquisition; Nucleic; Acid; Analyzer

An award is made to Kennesaw State University (KSU) to fund an electrophoretic nucleic acids analyzer and accessories for the use in multidisciplinary research and research training. Electrophoresis is the primary means of analyzing nucleic acids, separating nucleic acids based on their size and, to a lesser extent, conformation. Through an electrophoretic analysis, critical qualitative and quantitative information regarding the composition of a nucleic acid sample can be obtained. The nucleic acid analyzer will be part of the existing massively parallel / next generation sequencing facility, including an Ion Torrent Personal Genome Machine semiconductor sequencer (PI Van Dyke), thereby significantly expanding facility capabilities. Primary benefits will be in research, including projects involving undergraduate and graduate (Master of Science in Integrative Biology, Master of Science in Chemical Sciences) students. Additionally, programs that enhance research access to under-represented populations, including KSU's NIH-funded Peach State Bridges to the Doctorate and NSF-funded Chemistry and Biochemistry Summer Undergraduate Research Experiences programs, will benefit from this instrumentation. Involvement of the facility with course curricula is also planned, especially given the increasing importance of next-generation sequencing and transcriptome analysis in both health-related and research fields. Hands-on training in the technology used for contemporary nucleic acid sample quality control will better prepare KSU trainees for a broad range of STEM careers.An electrophoretic nucleic acids analyzer provides automated sample processing and scalable throughput for a variety of DNA and RNA quality control applications. For example, they are routinely used to determine the integrity of genomic DNA samples before their use in involved and costly procedures such as next generation sequencing, microarrays, and quantitative PCR. The nucleic acids analyzer will be used initially by a core group of five laboratories at KSU and their collaborators. Dr. Michael Van Dyke investigates orphan transcriptional regulators in a variety of organisms, including E. coli and Thermus thermophilus. Dr. Martin Hudson studies the role of heparan sulfate proteoglycans and anosmin-like proteins in nervous system development, using C. elegans and stem cell models of nervous system differentiation. Dr. Joel McNeal investigates photosynthetic gene evolution, host/parasite interactions, and microbial endophytes in the parasitic plant genus Cuscuta. Dr. Melanie Griffin studies the global targets of the leucine response protein in Pseudomonas aeruginosa. Dr. Marcus Davis explores the developmental mechanisms that pattern vertebrate appendages (fins and limbs) and the evolution of HOX gene regulation. Nucleic acids analysis will provide their materials, ranging from combinatorially selected fusion libraries to developmental stage-selected RNAs, with essential quality control assessments, thereby increasing the likelihood of success in their research and teaching endeavors.

授予肯尼索州立大学（KSU）一个奖项，资助一个电泳核酸分析仪和配件用于多学科研究和研究培训。电泳是分析核酸的主要手段，根据核酸的大小以及较小程度上的构象分离核酸。通过电泳分析，可以获得关于核酸样品组成的关键定性和定量信息。核酸分析仪将成为现有大规模并行/下一代测序设施的一部分，包括Ion Torrent Personal Genome Machine半导体测序仪（PI货车Dyke），从而显著扩展设施能力。主要的好处将是在研究，包括涉及本科生和研究生（综合生物学理学硕士，化学科学理学硕士）学生的项目。此外，提高对代表性不足的人群的研究机会的计划，包括KSU的NIH资助的桃州桥梁博士学位和NSF资助的化学和生物化学暑期本科生研究经验计划，将受益于这种仪器。还计划让该设施参与课程设置，特别是考虑到下一代测序和转录组分析在卫生相关领域和研究领域的重要性日益增加。现代核酸样品质量控制技术的实践培训将更好地为KSU学员提供广泛的STEM职业生涯。电泳核酸分析仪为各种DNA和RNA质量控制应用提供自动化样品处理和可扩展的吞吐量。例如，它们通常用于确定基因组DNA样品的完整性，然后用于涉及和昂贵的程序，如下一代测序，微阵列和定量PCR。核酸分析仪最初将由KSU的五个实验室及其合作者组成的核心小组使用。Michael货车Dyke博士研究了包括大肠杆菌在内的多种生物体中的孤儿转录调节因子。coli和嗜热栖热菌（Thermus thermophilus）。马丁哈德逊博士研究硫酸乙酰肝素蛋白聚糖和anosmin样蛋白在神经系统发育中的作用，使用C。神经系统分化的秀丽线虫和干细胞模型。Joel McNeal博士研究了寄生植物属菟丝子中的光合基因进化、宿主/寄生虫相互作用和微生物内生菌。Melanie Griffin博士研究铜绿假单胞菌中亮氨酸反应蛋白的全球目标。Marcus Davis博士探索了脊椎动物附属物（鳍和四肢）的发育机制和HOX基因调控的进化。核酸分析将为他们的材料提供必要的质量控制评估，从组合选择的融合文库到发育阶段选择的RNA，从而增加他们的研究和教学努力成功的可能性。