MRI: Acquisition of a BioSpectrum Advanced Imaging System for Interdisciplinary Research and Undergraduate Research Training

授予位于Geneseo的纽约州立大学一项合同，以获得UVP生物光谱815成像系统。这种先进的成像系统将通过提高数据收集的灵敏度来加速生物学、生物化学和化学领域的研究，并将允许在免疫学和细胞色素生物学领域扩大数据采集，细胞色素生物学是与蛋白质结合的含铁化合物的生物学。它将捕获和分析用紫外荧光或颜色试剂标签标记的蛋白质和DNA凝胶的高分辨率图像。此外，它将允许在同一凝胶上检查标记的核酸和蛋白质目标，并且还将对实验室生长的细菌和真核菌落进行成像。每年有30多名本科生将在学院领导的研究项目中使用该成像仪，每年有200多名本科生将在生态学、遗传学、分子和细胞生物学、免疫学、化学、生物化学、神经生物学和环境科学领域的实验室课堂作业中使用该成像仪。 UVP BioSpectrum 815成像系统将立即被纳入一些正在进行的项目，包括：（1）研究使用超强效类固醇对外阴癌发生的影响，这将揭示改变关键转录因子的机制;（2）研究布氏锥虫的核酸甲基化，这将解决缺乏对生物体中DNA碱基完整库的了解的问题;（3）研究控制视网膜生长和发育的分子和遗传机制，这导致对与视网膜发育缺陷相关的基因突变的理解;（4）研究应用于丝状真菌生长的主要减数分裂转录因子的转录和翻译调节;（5）研究C型细胞色素的生物起源和功能，进一步了解含铁或血红素蛋白质的合成;和（6）研究治疗性小分子与其核酸靶的相互作用，这将导致对分子相互作用的更好理解。预计生物学和化学系的其他研究人员以及SUNY Geneseo心理学系的至少一名研究人员将使用该仪器。该仪器将立即纳入生物学和化学系教授的几门实验室课程。

An award is made to the State University of New York at Geneseo to acquire a UVP BioSpectrum 815 imaging system. This advanced imaging system will accelerate research in the areas of Biology, Biochemistry and Chemistry through its increased sensitivity for data collection and will allow for expanded data acquisition in the areas of immunology and cytochrome biology, the biologics of iron-containing compounds bonded to a protein. It will capture and analyze high resolution images of protein and DNA gels that are labeled with UV fluorescent, or color reagent tags. In addition it will allow for examination of tagged nucleic acid and protein targets on the same gel and will also image laboratory-grown bacterial and eukaryotic colonies. More than 30 undergraduates per year will use the imager in faculty-led research projects and more than 200 undergraduates per year will utilize the imager in their laboratory classwork in the areas of ecology, genetics, molecular and cellular biology, immunology, chemistry, biochemistry, neurobiology and environmental sciences. The UVP BioSpectrum 815 Imaging System will be immediately incorporated into a number of ongoing projects including: (1) studies of the effects of ultrapotent steroid use on vulvar carcinogenesis that will shed light on the mechanisms involved in altering key transcription factors; (2) investigations into nucleic acid methylation in Trypanosoma brucei that will address a lack of knowledge on the full repertoire of DNA bases in organisms; (3) investigation into the molecular and genetic mechanisms controlling the growth and development of the retina, which is leading to an understanding of gene mutations associated with developmental defects of the retina; (4) investigation of the transcriptional and translational regulation of a major meiotic transcription factor as applied to growth of filamentous fungi; (5) investigations into the biogenesis and function of c-type cytochromes, furthering knowledge on the synthesis of iron-, or heme-containing proteins; and (6) studies into the interaction of therapeutically small molecules with their nucleic acid targets, which will lead to an improved understanding of molecular interactions. It is expected that additional investigators in the Biology and Chemistry Departments as well as at least one in the Psychology Department at SUNY Geneseo will utilize the instrument. The instrument will be immediately incorporated into several laboratory courses that are taught by both the Biology and Chemistry Departments.