Adaptations and counter-adaptations in the coevolutionary arms race of a baculovirus and its insect host

杆状病毒及其昆虫宿主共同进化军备竞赛中的适应和反适应

• 批准号: 226726063
• 负责人: Professor Dr. Johannes A. Jehle
• 金额: --
• 依托单位: Institut für biologischen Pflanzenschutz
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/226726063?language=en
• 关键词: Adaptations; counter; adaptations; coevolutionary; arms

Cydia pomonella granulovirus (CpGV, Baculoviridae) is one of the most important agents for the control of codling moth (CM, Cydia pomonella, L.) in both biological and integrated pest management. The rapid emergence of resistance against CpGV-M, which was observed in about 40 European CM field populations from 2003 on, could be traced back to a single, dominant, sex-linked gene. Since then, resistance management has been based on mixtures of new CpGV isolates (CpGV-I12, -S), which are able to overcome this resistance. Recently, resistance even to these novel isolates was observed in CM field populations. This resistance does not follow the described dominant, sex-linked inheritance trait. At the same time, another isolate CpGV-V15 was identified showing high virulence against these resistant populations. To elucidate this novel resistance mechanism and to identify the resistance gene(s) involved, we propose a comprehensive analysis of this resistance on the cellular and genomic level of codling moth. Because of the lack of previous knowledge of the molecular mechanisms of virus resistance in insects, several different and complementary approaches will be pursued. This study will not only give an in-depth insight into the genetic possibilities for development of baculovirus resistance in CM field populations and how the virus overcomes it, but can also serve as an important model for other baculovirus-host interaction systems.

苹果蠹蛾（Cydia pomonella，L.）在生物和综合虫害管理方面。从2003年开始，在大约40个欧洲CM田间种群中观察到的对CpGV-M的抗性的快速出现可以追溯到一个单一的、显性的、性连锁的基因。从那时起，耐药性管理一直基于新的CpGV分离株（CpGV-I12，-S）的混合物，这些分离株能够克服这种耐药性。最近，甚至在CM田间种群中观察到对这些新菌株的抗性。这种抗性不遵循所描述的显性、伴性遗传性状。同时，还鉴定了另一个对这些抗性群体表现出高毒力的分离物CpGV-V15。为了阐明这种新的抗性机制，并确定相关的抗性基因，我们提出了一个全面的分析，这种抗性的细胞和基因组水平的苹果蠹蛾。由于缺乏对昆虫病毒抗性的分子机制的先前知识，将追求几种不同的和互补的方法。这项研究不仅将深入了解CM田间种群中杆状病毒抗性发展的遗传可能性以及病毒如何克服它，而且还可以作为其他杆状病毒-宿主相互作用系统的重要模型。