Role of bioactive lipid signaling in homeostatic control of excitatory transmission. (B05)

在即将到来的资金阶段，我们将1)使用电生理和行为方法来研究PRG-1/CaM结合的作用，它似乎是动态调节的。2)进一步分析星形胶质细胞突触周围板层LPA合成酶ATX在兴奋性突触中的作用。3)最后，我们将使用时间分辨率在毫秒范围内的单刺2P成像来评估树突棘对LPA的摄取和由此产生的钙瞬变。这将允许确定动力学，并得出突触磷脂功能的定量数学模型。这些数据将为生物活性脂质对突触信号的稳态控制提供一个新的视角。

In the upcoming funding period, we will 1) address the role of PRG-1/CaM binding, which appears to be dynamically regulated, using electrophysiological and behavioral methods. 2) Further, we will analyze the role of the LPA-synthesizing enzyme ATX, present at perisynaptic lamellae of astrocytes, in excitatory synapses. 3) Finally, we will assess LPA-uptake and resulting Ca2+-transients in dendritic spines using single-spine 2P-imaging with a temporal resolution in the milliseconds range. This will allow to determine kinetics and to arrive at a quantitative mathematical model for synaptic phospholipid function. These data will provide a new view on the homoeostatic control of synaptic signaling by bioactive lipids.