I-Corps: Colorimetric sensors for the detection of volatile amine

I-Corps：用于检测挥发性胺的比色传感器

• 批准号: 1661642
• 负责人: Javier Read de Alaniz
• 金额: $ 5万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-11-01 至 2018-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1661642&HistoricalAwards=false
• 关键词: Corps; Colorimetric; sensors; detection; volatile

The broader impact/commercial potential of this I-Corps project is advancement of sensing technology for health, food safety, and diagnostics, and taps into the rapidly growing global market for sensors. Colorimetric sensors are a family of compounds that undergo a conversion between two different colored states upon exposure to a stimulus and are validated in real-world applications such as blood-glucose monitoring and early pregnancy tests. This project focuses on the development and deployment of simple and cost effective colorimetric indicators for use in applications such as detection of food spoilage, detection of contaminants in water, detection of pharmaceutical residues, monitoring wounds for the onset of infection, and tracking solid phase peptide and peptoid synthesis. The initial focus area applies to the large market, where these indicators can be used to determine the freshness of meat and seafood, and can help address the problems of food waste and food-borne illnesses.This I-Corps project is based on a growing interest in smart materials ? compounds that change properties or state as a result of an external input. In particular, this research is based on the development of a portable and inexpensive colorimetric sensor capable of detecting sub-ppm levels of amine in the presence of thiols and alcohols, and prepared from low-cost, commercially available starting materials. The reaction between an activated furan and these low levels of amines, in solution or when incorporated onto solid supports, produces a vibrant pink color under ambient conditions that is distinguishable by the naked eye or by using the digital camera of a smartphone. Analysis of the kinetics of these reactions in solution and the quantification of the levels of detectable amine show excellent sensitivity and selectivity. In application, activated furan solutions were used as a colorimetric assay for solid-phase synthesis of peptides and peptoids and were adsorbed onto nylon filters and used to monitor the real-time release of amines from decaying fish samples.

这个I-Corps项目的更广泛的影响/商业潜力是健康，食品安全和诊断传感技术的进步，并进入快速增长的全球传感器市场。比色传感器是一系列化合物，它们在暴露于刺激物时经历两种不同颜色状态之间的转换，并在血糖监测和早孕测试等实际应用中得到验证。该项目的重点是开发和部署简单且具有成本效益的比色指示剂，用于检测食品腐败，检测水中污染物，检测药物残留，监测伤口感染的发生，以及跟踪固相肽和类肽合成等应用。最初的重点领域适用于大型市场，这些指标可用于确定肉类和海鲜的新鲜度，并有助于解决食物浪费和食源性疾病的问题。由于外部输入而改变性质或状态的化合物。特别是，这项研究是基于一种便携式和廉价的比色传感器的发展，能够检测亚ppm水平的胺在硫醇和醇的存在下，并从低成本，市售的原料制备。在溶液中或当掺入到固体载体上时，活化的呋喃与这些低水平的胺之间的反应在环境条件下产生鲜艳的粉红色，其可通过肉眼或通过使用智能手机的数码相机区分。这些反应在溶液中的动力学分析和可检测的胺的水平的定量显示出优异的灵敏度和选择性。在应用中，活化的呋喃溶液被用作肽和类肽的固相合成的比色测定，并被吸附到尼龙过滤器上，用于监测从腐烂的鱼样品中实时释放的胺。