EAPSI: Investigating the Distribution and Diversity of Anaerobic Ammonium Oxidizing Bacteria in a Geothermal Hot Spring Ecosystem

EAPSI：研究地热温泉生态系统中厌氧氨氧化细菌的分布和多样性

• 批准号: 1713831
• 负责人: Jennifer Lawrence
• 金额: $ 0.54万
• 依托单位: Lawrence Jennifer E
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1713831&HistoricalAwards=false
• 关键词: EAPSI; Investigating; Distribution; Diversity; Anaerobic

This project will investigate the abundance, distribution, and functional capabilities of anaerobic ammonium oxidizing (anammox) bacteria in a geothermal hot spring ecosystem located on the North Island of New Zealand. Anammox bacteria play an important role in removing reactive nitrogen (i.e., nitrogen species that directly or indirectly support growth) from both natural and engineered ecosystems, but their role in removing reactive nitrogen from geothermal hot spring ecosystems is not well understood. Increased knowledge of anammox bacteria is particularly valuable today, because most of the world's industrialized nations are struggling to manage elevated concentrations of reactive nitrogen in their fresh and marine water bodies. The insights into the distribution and diversity of anammox bacteria gained as a result of this project will help scientists and engineers develop and apply more robust and sustainable reactive nitrogen removal processes. This project will be conducted in collaboration with Dr. Wei-Qin Zhuang, a noted expert in environmental microbiology and biotechnology, at the University of Auckland in New Zealand. Geothermal hot spring ecosystem samples will be collected from the One Thousand Springs database, which has catalogued the 16S rRNA microbial diversity and associated geochemical conditions of over 1,000 geothermal hot spring ecosystem samples collected from the Taupo Volcanic Zone on New Zealand's North Island. This database will be mined to identify samples that indicate the existence of anammox bacteria. Further analyses will be performed on these samples to elucidate the abundance and functional capabilities of anammox bacteria within these samples. Abundance will be determined through quantitative polymerase chain reaction analyses, and functional capabilities will be determined through metagenomic analyses. Subsequently, these same analyses will be performed on samples extracted from geothermal hot spring enrichment bioreactors operated by the Zhuang Laboratory, in order to understand the impacts of the translation of extremophilic anammox bacteria from a natural ecosystem to an engineered bioreactor.This award, under the East Asia and Pacific Summer Institutes program, supports summer research by a U.S. graduate student and is jointly funded by NSF and the Royal Society of New Zealand.

该项目将调查新西兰北岛地热温泉生态系统中厌氧氨氧化（anammox）细菌的丰度、分布和功能。厌氧氨氧化细菌在从自然和工程生态系统中去除活性氮（即直接或间接支持生长的氮物种）方面发挥着重要作用，但它们在从地热温泉生态系统中去除活性氮的作用尚不清楚。如今，增加对厌氧氨氧化细菌的了解尤其有价值，因为世界上大多数工业化国家都在努力管理其淡水和海洋水体中活性氮浓度升高的问题。通过该项目获得的对厌氧氨氧化细菌的分布和多样性的见解将帮助科学家和工程师开发和应用更强大和可持续的反应性脱氮工艺。该项目将与新西兰奥克兰大学环境微生物学和生物技术领域的著名专家庄伟勤博士合作进行。 地热温泉生态系统样本将从千泉数据库采集，该数据库对从新西兰北岛陶波火山区采集的 1000 多个地热温泉生态系统样本的 16S rRNA 微生物多样性和相关地球化学条件进行了分类。该数据库将被挖掘以识别表明厌氧氨氧化细菌存在的样本。将对这些样品进行进一步分析，以阐明这些样品中厌氧氨氧化细菌的丰度和功能。丰度将通过定量聚合酶链反应分析确定，功能能力将通过宏基因组分析确定。随后，将对从庄实验室运行的地热温泉富集生物反应器中提取的样本进行这些相同的分析，以了解极端厌氧氨氧化细菌从自然生态系统转化为工程生物反应器的影响。该奖项属于东亚和太平洋暑期学院项目，支持一名美国研究生的夏季研究，并由以下机构共同资助： NSF 和新西兰皇家学会。