EAPSI: Investigating Stem Elongation as a Means to Increase Crop Productivity

EAPSI：研究茎伸长作为提高作物生产力的一种手段

• 批准号: 1714067
• 负责人: Caitlin Jacques
• 金额: $ 0.54万
• 依托单位: Jacques Caitlin
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1714067&HistoricalAwards=false
• 关键词: EAPSI; Investigating; Stem; Elongation; Means

As sessile organisms, plants need to effectively sense and respond to their environment in order to survive. One way they accomplish this is through the perception and response to light. A key group of proteins involved in modulating light-mediated responses are the AHLs, a group of DNA-binding proteins that influence stem elongation in seedlings. Stem elongation is among the first crucial steps in plant development for many crop plants. As the climate continues to change, it is increasingly important for us to gain a better understanding of how stem elongation is controlled, as it will facilitate the improvement of crop varieties that can cope with such changes. This EAPSI fellowship will enable further investigation into the role AHLs play in stem elongation, and how this elongation is further regulated by plant hormones. This research will be conducted at the RIKEN Institute under the direction of Dr. Keiko Sugimoto, a leading expert in plant cell research.Using Arabidopsis as a model system, the overarching goal of this research is to understand how light mediates seedling growth. One key group of genes involved in this process is the AT-HOOK MOTIF NUCLEAR LOCALIED (AHL) gene family. AHL29/SUPRESSOR OF PHYTOCHROME B-4 (SOB3) directly regulates the auxin-associated genes YUCCA8 (YUC8) and members of the SMALL AUXIN UP RNA 19 (SAUR19) subfamily during seedling development. SOB3 is able to modulate hypocotyl elongation in seedlings, at least in part, by repressing transcription of the aforementioned genes. Since YUC8 and members of the SAUR19 subfamily are directly involved in phytohormone pathways, ChIP-Seq will be used to test the hypothesis that SOB3 binds to the promoters of additional genes associated with plant-specific hormone pathways, such as gibberellic acid, cytokinins, and jasmonic acid. Additionally, hypocotyl elongation assays will be performed using sob mutants and the aforementioned hormones to further investigate these interactions.This award, under the East Asia and Pacific Summer Institutes program, supports summer research by a U.S. graduate student and is jointly funded by NSF and the Japan Society for the Promotion of Science.

作为固着生物，植物需要有效地感知和响应环境才能生存。它们实现这一点的一种方式是通过对光的感知和反应。参与调节光介导反应的一组关键蛋白质是AHLS，这是一组影响幼苗茎伸长的DNA结合蛋白。茎伸长是许多农作物植株发育的最初关键步骤之一。随着气候的持续变化，更好地了解茎伸长是如何控制的，这对我们来说越来越重要，因为这将促进能够应对这种变化的作物品种的改良。这项EAPSI奖学金将使人们能够进一步研究AHL在茎伸长中所起的作用，以及这种伸长如何进一步受到植物激素的调节。这项研究将在理研研究所进行，在植物细胞研究领域的领军人物杉本惠子博士的指导下进行。以拟南芥为模型系统，本研究的首要目标是了解光如何调节幼苗的生长。参与这一过程的一组关键基因是AT-HOOK基序核锁基因(AHL)基因家族。光敏色素B-4(SOB3)的AHL29/SUPRESSOR基因在幼苗发育过程中直接调控生长素相关基因YUCCA8(YUC8)和小生长素上调RNA19(SAUR19)亚家族成员。SOB3至少部分通过抑制上述基因的转录来调节幼苗下胚轴的伸长。由于YUC8和SAUR19亚家族的成员直接参与植物激素途径，CHIP-SEQ将被用来检验SOB3与与植物特定激素途径相关的额外基因的启动子结合的假设，如赤霉酸、细胞分裂素和茉莉酸。此外，下胚轴伸长分析将使用SOB突变体和上述激素进行，以进一步研究这些相互作用。该奖项由东亚和太平洋夏季学院计划资助一名美国研究生的夏季研究，由NSF和日本科学促进会共同资助。